微生物法测定乳粉中维生素B_(12)的应用和探讨  

Application and discussion of microbiological method for assay of Vitamin B_(12) in milk powder

作　　者：张丽宏[1] 王克新[1] 王淼[1] 房玉国[1] 王旻[1] 卢雁[1] 

机构地区：[1]东北农业大学国家乳制品质量监督检验中心,哈尔滨150086

出　　处：《中国乳品工业》2008年第10期58-60,共3页China Dairy Industry

摘　　要：国家标准GB/T 5413.14-1997等同采用AOAC 986.23微生物法,是目前用于维生素B12测定的主要方法和仲裁方法,但该法操作复杂,影响结果的因素很多,对检验人员和实验室的要求较高。现将测定中关键步骤,主要影响因素,容易出现的问题等进行探讨,包括提取方法、标线制备、防止污染、菌种保存、注意事项及操作一致性的必要性等诸多方面,可作为对该方法的说明和补充。Equivalent to microbiological Method in AOAC 986.23, Chinese national standard GB/T 5413.14-1997 is currently the main and arbitrary method for assay of vitamin B12, while the method raise relatively high demand for laboratory and analyst for its complicated operation and much influencing factors. The article discusses into critical seeps, main disturbance and existing problems including extracting method, preparation of standard solution, prevention from contamination, cautions for proliferation and maintenance of strains, and necessity for keeping consistence in assay operation, it may be considered as explanation and supplement for the assay method.

关 键 词：微生物法 维生素B 测定 探讨 关键步骤 影响因素 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]