拟南芥网状突变体E-210基因精细定位  

Fine Mapping of the Reticula Mutant E-210 Gene in Arabidopsis thaliana

作　　者：宋磊[1] 杨仲南[1] 吴世福[1] 崔永兰[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《武汉植物学研究》2008年第5期437-442,共6页Journal of Wuhan Botanical Research

基　　金：上海市高校选拔培养优秀青年教师科研专项基金(RE567)资助

摘　　要：通过甲基磺酸乙酯(EMS)诱变与遗传分析,从拟南芥(Arabidopsis thaliana)中筛选到一株隐性单基因控制的网状突变体E-210。该突变体植株生长缓慢,叶脉呈绿色,叶肉呈黄色。通过透射电镜观察,发现野生型植株和突变植株在叶绿体结构上差异不大,猜测该突变体E-210基因与叶绿体的发育可能没有直接关系,而很可能同叶绿素或叶绿体的生物合成有关。通过图位克隆的方法,将该突变体的突变基因定位在第5条染色体上的MRB17和MBG8-5的分子标记之间,精确到87.130kb。对MRB17和MBG8-5的分子标记之间的22个基因进行了分析,预测突变体E-210基因可能是At5g54770,编码THI1,即噻唑合成酶。Seeds of the Arabidopsis thaliana ecotype Landsberg erceta were chemically mutagenized with ethyl-methylsulfonate （EMS） and the M2 progeny screened for seedlings with chloroplast-related mutations affecting leaf coloration. An independent mutant line of reticula controlled by a novel nuclear gene, designated E-210, was generated and identified. Genetics analysis indicates that the mutant was controlled by a single recessive gene E-210. Phenotypic analysis indicates that the reticula mutant grows up more slowly than the wild-type plant and typically has a green vasculature on yellow lamina. Transmission electron microscopic examination shows that the chloroplasts in the mutant are not obviously different from ones in the wild-type plant. We suppose that the gene for E-210 mutant is not directly related to chloroplast development, but probably related to chlorophyll or chloroplast biosynthesis. We have mapped E-210 to a region of 87. 130 kb between the molecular markers MRB17 and MBG8-5 on chromosome 5 using map-based cloning technique. We propose AtSg54770 encoding TH1 ,the thizaole biosynthesis enzyme,is responsible for the phyenotype of E-210 mutant by analysis of 87. 130 kb.

关 键 词：拟南芥 图位克隆 精细定位 透射电镜技术(TEM) 基因预测 

分 类 号：Q943[生物学—植物学]