泊洛沙姆(Poloxamer)在脂溶性化感物质生物检测中的应用  

Application of Poloxamer in Fat-soluble Allelochemicals Bioassay

作　　者：陶月红[1] 张昭[1] 张本刚[1] 斯建勇[1] 

机构地区：[1]中国医学科学院北京协和医科大学药用植物研究所,北京100094

出　　处：《武汉植物学研究》2008年第5期533-539,共7页Journal of Wuhan Botanical Research

基　　金：国家中医药管理局科学技术研究专项(04-05ZP07)"濒危中药材关黄柏野生资源抚育基础研究";北京市教委重点学科共建项目(XK100230448)"濒危药用植物黄檗野生种群自我更新障碍机制研究"

摘　　要：在脂溶性化感物质的生物检测中,常规方法是使用有机溶剂对化感物质进行助溶,此法本身会对受体植物产生为害,并且不能准确定量。本研究结合药剂学理论,将动物和细胞实验中常用的增溶剂泊洛沙姆(F-68)应用于脂溶性化感物质的生物检测,用泊洛沙姆对香草醛和阿魏酸两种化感物质进行增溶,以乙醇助溶为对照,比较两种方法的化感物质活性。结果表明,化感物质用泊洛沙姆增溶后,形成的溶液是一个稳定的体系,试验过程中浓度不会发生变化,可准确定量考察化感物质的活性,同时泊洛沙姆对受体植物不产生影响。乙醇助溶处理中,或由于乙醇不易完全挥发,因乙醇的存在而对受体植物产生影响,表现为化感物质活性的加强;或随乙醇的挥发,化感物质以晶体析出,表现为化感物质活性的减弱,不能准确定量考察化感物质的活性。During bioassay for the fat-soluble allelochemicals, we generally use common organic solvents such as ethanol etc. to solubilize the fat-soluble substances, for the method, it easily causes toxic effects on seeds and influence accuracy of the bioassay. On the principle of pharmacy, poloxamer （F-68） is adopted in our research,which is a kind of surfactant used in animal and cytological experiments, as a solubilizer to the experiment of fat-soluble alleloehemicals bioassay. We compared the activity of vanillin and ferulic acid with two methods of the poloxamer and ethanol solution in general. The result showed that the concentration of allelochemicals solution processed by poloxamer had no change in quantitative detection of allelopathy activity, and no harmful effect on seed germination and seedling growth, and making for quantitative studies to the activity of allelochemicals. In contrast with ethanol groups, we found that the activity of allelochemicals has been strengthened, or weakened which caused by over concentration from ethanol volatile, to the extent that the allelochmicals crystallized from the solution. The research indicated that the method which the allelochemicals processed by poloxamer has adventages than the general method in fat-soluble allelochemicals.

关 键 词：化感作用 生物检测 泊洛沙姆 香草醛 阿魏酸 

分 类 号：Q-331[生物学]