Raf激酶抑制蛋白和P-ERK在肝细胞癌中的表达及其临床意义  

Expressions of Raf kinase inhibitor protein and P-ERK in hepatocellular carcinoma and their significance

作　　者：吴晓慧[1] 王顺祥[1] 杨永江[1] 李建坤[1] 彭利[1] 唐瑞峰[1] 王士杰[2] 

机构地区：[1]河北医科大学第四医院肝胆外科,石家庄050011 [2]河北医科大学第四医院肿瘤研究所,石家庄050011

出　　处：《肿瘤》2008年第10期869-872,共4页Tumor

摘　　要：目的:探讨Raf激酶抑制蛋白(Raf kinase inhibitor protein,RKIP)和磷酸化细胞外信号调节激酶(phos-extracellular signal regulated kinase,P-ERK)在肝细胞癌(hepatocellular carcinoma,HCC)中的表达及其临床意义。方法:应用免疫组织化学方法检测RKIP和P-ERK在HCC、癌旁及正常肝组织中的表达,并分析其与HCC临床病理学特征的关系。结果:HCC组织中RKIP的表达低于癌旁及正常肝组织,而P-ERK的表达高于癌旁及正常肝组织,差异有统计学意义;HCC组织中RKIP与P-ERK的表达呈显著负相关(r=-0.227,P=0.039);RKIP在HCC组织中的表达与有无肝内或淋巴结转移及肿瘤分化程度相关(P<0.05);P-ERK在HCC组织中的表达与肿瘤分化程度、有无癌栓及有无肝内或淋巴转移相关(P<0.05)。结论:RKIP表达的减少或缺失与HCC的发生、发展密切相关,RKIP表达的减少或缺失可能通过上调P-ERK的表达促进HCC的侵袭和转移。Objective：To investigate the expression and clinical significance of Raf kinase inhibitor protein （RKIP） and phos-phorylated-extracellular signal regulated kinase （P-ERK） in hepatocellular carcinoma （HCC） and their correlations with the invasion and metastasis of HCC. nethods：Immunohistochemistry was used to detect the expressions of RKIP and P-ERK in HCC tissues, paracancerous tissues, and normal liver tissues. Statistical analysis was used to determine the relationship between their expressions and clinicopathological parameters. Results： Statistical analysis revealed that RKIP-positive staining rate was lower in tumor tissues than that in tumor-surrounding tissues and in normal liver tissues. But P-ERK-positive staining rate was significantly higher in tumor tissues than that in tumor-surrounding tissues and normal liver tissues. The difference was significant. RKIP expression had negative correlation with P-ERK expression （r = - 0. 227, P = 0.039） ; RKIP expression in HCC was associated with intrahepatic or lymphatic metastasis and tumor differentiation （ P 〈 0.05 ）. P-ERK expression in HCC was related with tumor differentiation, tumor thrombosis, and intrahepatic or lymphatic metastasis （ P 〈 0.05 ）. Conclusions： These findings indicate that down-regulation or loss of RKIP expression is closely related with HCC development and progression. The down-regulation or loss of RKIP expression stimulates migration and metastasis of HCC by up-regulating P-ERK expression.

关 键 词：癌 肝细胞 RAF激酶抑制蛋白 细胞外信号调节激酶 转移 

分 类 号：R735.7[医药卫生—肿瘤]