血竭素高氯酸盐对早期糖尿病肾病肾脏损伤防治作用的研究  

To study of the protective and therapeutic effect of dracorhodin perchlorate on renal injury of early diabetic nephropathy

作　　者：王全胜[1] 张丽晓[1] 刘建国[1] 汪永辉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科,湖北武汉430022

出　　处：《中国药理学通报》2008年第10期1331-1334,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30600810)

摘　　要：目的探讨血竭素高氯酸盐对早期糖尿病肾病的肾脏保护作用。方法采用链脲佐菌素(streptozotocin,STZ)150mg·kg-1单次腹腔注射C57BL/6小鼠,建立小鼠早期糖尿病肾病模型,设正常对照组、糖尿病肾病模型组、胰岛素对照组及血竭素高氯酸盐5、10、20mg·kg-1.d-13种剂量治疗组(血竭素高氯酸盐溶于生理盐水中灌胃)。治疗4wk后检测各组小鼠肾重指数、肾小球细胞外基质(extracellular ma-trix,ECM)、24h尿蛋白含量、肌酐清除率,用RT-PCR方法检测纤连蛋白(fibronectin,FN)mRNA表达,免疫组化方法检测FN蛋白的表达及分布。结果与糖尿病肾病模型组比较,血竭素高氯酸盐治疗组的肌酐清除率及尿蛋白均降低(P<0.05);各治疗组均能减少肾皮质中肾小球ECM的积聚(P<0.05)、FN mRNA和蛋白的表达(P<0.01)。结论血竭素高氯酸盐能保护早期糖尿病肾病的肾损伤。Aim To study the protective effect of dracorhodin perchlorate on early diabetic nephropathy. Methods 8 weeks old C57BL/6 male mice were randomly divided into six groups：normal control;diabetic nephropathy control;disease mice administered with insulin;disease mice administered with dracorhodin perchlorate at the concentration of 20,10, and 5 mg · kg^ - 1 · d^-1. Diabetic nephropathy mice were induced by streptozotocin （STZ） 150 mg · kg^-1 peritoneal injection. The mice were sacrificed at the end of the 4 th week and metabolic data were measured at 4 th week. Pathological change of glomerulal extracellular matrix （ECM） in renal cortex in six groups was detected by PAS staining, and the expression of fibronectin （FN） in renal cortex was detected by RT-PCR and immunohistochemistry. Results Dracorhodin perchlorate could reduce significantly the expression of FN and extracellular matrix （ECM） in renal cortex, and decrease significantly GFR （ glomerular filtration rate ） and proteinuria. The reduction is dose-dependent on dracorhodin perchlorate treatment. Conclusion Dracorhodin perchlorate had protective effect on renal injury of early diabetic nephropathy.

关 键 词：血竭素高氯酸盐 糖尿病肾病 纤连蛋白 肾损伤 

分 类 号：R-332[医药卫生] R282.71