大鼠角膜层间植入微粒体诱导新生血管模型的研究(英文)  

Angiogenesis induced by micropocket assay on rat cornea

作　　者：赵伟[1] 蒋爱华[2] 刘祖国[2] 

机构地区：[1]广州市儿童医院眼科,中国广东省广州市520120 [2]厦门大学眼科中心,中国福建省厦门市361005

出　　处：《国际眼科杂志》2008年第10期1959-1962,共4页International Eye Science

基　　金：National Science Fund for Distinguished Young Scholars(No.30225044);Fund for Innovative Research Groups of China(No.30321004);Science Foundation of Board of Health of Guangzhou(No.2008-YB-053)~~

摘　　要：目的:探讨大鼠角膜层间植入微粒体诱导新生血管模型的制作技巧和特点。方法:9只SD大鼠手术显微镜下做角膜基质微囊袋并植入含有VEGF的缓释微粒体。术后1、3、5、7d采用裂隙灯显微镜观察角膜新生血管的生长情况。结果:大鼠角膜微囊袋内植入微粒体后1d仅见角膜缘血管网充血扩张,未见新生血管生长;3d可见毛刷状新生血管自角膜缘伸入角膜,面积为(2.23±0.59)mm2;5d新生血管垂直于角膜缘向微粒体方向束状延伸,并达到微粒体下缘部位,面积为(6.81±1.35)mm2;7d新生血管继续向前生长,达到并超过微粒体下缘,部分新生血管顶端互相吻合成袢状,面积为(8.92±1.79)mm2。其中,5d和7d新生血管面积比较无显著性差异。所有术眼均未见前房积血、角膜溃疡、微粒体脱出等并发症。结论:大鼠角膜层间植入微粒体诱导的新生血管生长稳定、无并发症、适用于定量研究。AIM： To explore the skills and characteristics of corneal neovascular model in rat induced by micropocket assay. METHODS- Nine eyes of nine Sprague-Dawley rats were studied. Pellets made of vascular endothelial growth factor （VEGF）, poly-2-hydroxylethylmethacrylate and sucralfate were implanted into the corneal stroma no closer than l mm from the limbus. Biomicroscopic features of corneal neovascular were observed on 1,3, 5, 7th day after the implantation. RESULTS： On day 1 after operation, the limbal vessels were dilated, with no angiogenesis appeared. On day 3, angiogenesis began to invade peri-cornea with a brush shape, the area of CNV was （2.23±0.59）mm^2. On day5, new vessels reached the lower margin of pellet densely, the area of CNVwas （6.81 ±1.35）mm^2, On day 7, new vessels continued to elongate, parts of them extended as loops toward the pellet, the area of CNV was （8. 92±1.79） mm^2. Neither hyphema or other complications occurred. CONCLUSION： Corneal neovascular induced by micropocket assay in rat grows steadily, with no complication, and is suitable for quantitative researches.

关 键 词：新生血管 微囊袋法 血管内皮细胞生长因子 

分 类 号：R772[医药卫生—眼科] R-332[医药卫生—临床医学]