基质细胞衍生因子-1α对体外培养人视网膜色素上皮细胞增殖的影响研究  

Effect of stromal cell-derived factor-1α on the proliferation of human retinal pigment epithelium in vitro

作　　者：徐国兴[1] 陈瑾[1] 郑学栋[1] 郭健[1] 谢茂松[1] 

机构地区：[1]福建医科大学附属第一医院眼科,福建省眼科研究所,中国福建省福州市350005

出　　处：《国际眼科杂志》2008年第10期2001-2004,共4页International Eye Science

基　　金：中国卫生部科研基金资助项目(No.WKJ2005-2-013);福建省重大科研项目(No.002Y002);福建医科大学教授发展基金课题(No.2006-js6033)~~

摘　　要：目的:研究基质细胞衍生因子-1α(stromal cell-derived fac-tor-1α,SDF-1α)对体外培养的人类视网膜色素上皮(hu-man retinal pigment epithelium,hRPE)细胞增殖的影响。方法:应用MTT法研究SDF-1α对体外培养的人RPE细胞增殖的影响;用细胞化学染色法检测添加SDF-1α前后人视网膜色素上皮细胞内细胞增殖核抗原(proliferatingcell nucler antigen,PCNA)的表达情况。结果:MTT法表明SDF-1α可促进体外培养的hRPE细胞的增殖(P<0.05);细胞化学电镜下观察发现:添加SDF-1α前hRPE细胞未表达或低表达PCNA,而添加SDF-1α后细胞高度表达PCNA。结论:细胞因子SDF-1α可促进体外培养的hRPE细胞增殖。AIM： To investigate the effect of stromal cell-derived factor-It（（SDF-la） on the proliferation of human retinal pigment epithelium （hRPE） in vitro. METHODS： MTT was used to investigate the effect of SDF-la on the proliferation of hRPE in vitro. Cytochemical staining method was used to detect the expression of proliferating cell nuclear antigen （PCNA） in hRPE with SDF-1α. RESULTS. MTT showed that SDF -1α could stimulate the proliferation of hRPE in vitro（ P〈 0. 05）, Cytochemistry results indicated that there were no or low expression of PCNA before adding SDF-1α, but the expression became higher after adding SDF-1α observed under electric mirror. CONCLUSION. Cytokine SDF-1α can stimulate the proliferation of hRPE cultured in vitro.

关 键 词：人视网膜色素上皮细胞 基质细胞衍生因子-1Α 体外培养 增殖 

分 类 号：R774.1[医药卫生—眼科]