TRIS饱和酚一步法提取食用菌DNA  

Research on the TRIS Saturated Phenol Method of DNA Extraction from Edible Fungus

作　　者：赵春喜 马利华[2] 杨同文[2] 

机构地区：[1]郑州市卫生学校,河南郑州450005 [2]周口师范学院,河南周口466000

出　　处：《安徽农业科学》2008年第28期12122-12122,12200,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为探索食用菌子实体DNA的快速提取方法。[方法]以4种广泛栽培的食用菌子实体为材料,采用改进的TRIS饱和酚法提取食用菌基因组DNA,并用琼脂糖凝胶电泳和PCR技术检测提取DNA样品的质量。[结果]获得的食用菌DNA质量较好,能够满足PCR等后续分子生物学试验。[结论]TRIS饱和酚法是一种简单、快速提取食用菌DNA的方法。[ Objective] The extraction method of DNA from edible fungus was developed. [ Method] Four types of fungi, which were widely cultured, being taken as testing material, the improved TRIS saturated phenol extraction method was applied and the quality of extracted DNA was examined with agarose gel electrophoresis and PCR technique in the experiment. [ Results] The good-quahty DNA could be extracted, which can meet the demand in the follow-up molecular research. [Conelusion] The improved method was one kink of easy and rapid practical method.

关 键 词：食用菌 TRIS饱和酚法 DNA提取 

分 类 号：S646[农业科学—蔬菜学]