siRNA阻断NF-κB信号通路抑制宫颈癌HeLa229细胞的增殖及侵袭  

Knockdown of NF-κB signaling pathway by siRNA inhibits the proliferation and invasiveness of HeLa229 cells

作　　者：田卫红[1] 田芳[2,3] 许培荣[3] 刘红涛[3] 薛乐勋[3] 

机构地区：[1]河南省安阳市肿瘤医院妇科肿瘤,河南安阳455000 [2]郑州大学基础医学院病理生理学教研室,河南郑州450052 [3]郑州大学基础医学院细胞生物学研究室,河南郑州450052

出　　处：《基础医学与临床》2008年第10期1044-1047,共4页Basic and Clinical Medicine

基　　金：教育部"十五"211工程重点建设项目:肿瘤与生物工程(教重办[2002]第2号)

摘　　要：目的通过RNA干扰阻断宫颈癌HeLa229细胞中NF-κB信号通路,研究其与肿瘤细胞增殖、耐药和侵袭力的关系。方法利用RNAi技术,将HeLa229分为转染组和对照组,MTT法检测细胞增殖,Boyden chamber体外侵袭实验检测细胞体外侵袭力。结果MTT实验表明,转染组细胞存活率比对照组明显下降,联合应用化疗药,转染组和对照组细胞的存活率均随着5-Fu浓度的增加而下降,但在同一浓度,siRNA与5-Fu联合应用可明显提高HeLa229细胞对化疗药的敏感性。体外侵袭实验结果表明,和对照组相比,转染P65siRNA组穿越Matrigel胶的细胞数明显减少(P<0.05)。结论应用RNAi技术可有效阻断NF-κB信号通路,抑制宫颈癌细胞的增殖和体外侵袭力,增强对5-Fu的敏感性。因此,可将NF-κB信号通路作为宫颈癌基因治疗的靶点。Objective To investigate cell proliferation and invasiveness of cervical cancer HeLa229 cell after knockdown of NF-κB signaling pathway by P65 siRNA. Methods RNA interference was employed for specific in hibition of the expression of P65. HeLa229 cell was divided into transfected group and untransfected group. Cell viability was detected by MTT after the HeLa229 cells were transfected with or without P65 siRNA for 24, 48, 72 h. The sensitivity to 5-Fu of the HeLa229 cell, transfected with or without P65 siRNA, was evaluated also by MTT. Boyden chamber experiment in vitro was used to detect the invasion of HeLa29 cell. Results P65 siRNA inhibited the cell proliferation as compared with the untransfected cells. Proliferations of both cells transfected with and without P65 siRNA were inhibited in a concentration-dependent manner, while at the same concentration of 5-Fu the viability of transfected HeLa229 cells was significantly suppressed （ P 〈 0. 05 ）. Compared with the un- transfected cells, the number of cells which traversed through Matrigel was decreased obviously. Conclusion RNAi targeting of P65 has anti-proliferative effects, inhibits the invasiveness and increases the 5-Fu sensitivity of the ESCC cells, suggesting that NF-κB might be a target for cancer treatment.

关 键 词：宫颈癌 NF-KAPPAB RNA干扰 SIRNA 

分 类 号：R737.33[医药卫生—肿瘤]