致病疫霉菌无毒基因AVR3a的克隆与序列分析  

Cloning and sequence analysis of AVR3a gene in Phytophthora infestans

作　　者：兰成忠[1,2] 陈庆河[2] 汪进仕[2] 胡方平[1] 李本金[2] 赵健[2] 邱荣洲[2] 翁启勇[2] 

机构地区：[1]福建农林大学植物保护学院,福建福州350002 [2]福建省农业科学院植物保护研究所,福建福州350013

出　　处：《福建农业学报》2008年第3期235-238,共4页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30600401);农业部公益性行业(农业)科研专项(nyhyzx07-053);福建省农业科学院青年科技人才创新项目(闽农科院发[2006]157号);福建省财政专项-福建省农业科学院科技创新团队建设基金(STIF-Y07)

摘　　要：根据GenBank中致病疫霉菌无毒基因AVR3a序列(AJ893357)及参考文献中的特异引物(Pex147F,Pex147R),以致病疫霉菌基因组DNA为模板进行PCR扩增,得到了特异性扩增片段,并对特异片段进行纯化和测序。结果表明,该片段的长度为451 bp,经序列分析表明,其氨基酸编码序列为441 bp,共编码147个氨基酸。BLAST分析结果显示,该片段核苷酸序列与无毒基因AVR3a核苷酸序列的同源性为99.34%,氨基酸序列比对结果表明,氨基酸序列在第19、80和103位存在着差异,这些核苷酸序列差异以及某些关键氨基酸的改变有可能是导致不同菌株中AVR3a毒性变异的原因。Based on the AVR3a DNA sequence for Phytophthora infestans found in GenBank （accession No. AJ893357） and its primers （Pex147F, Pex147R） reported in references, PCR was carried out with the genomics DNA of P. infestans as template. The specific fragment was purified and cloned. The results showed that the fragment was 451bp. And, the analysis of sequence indicated that the coding region was 441bp and encoded 147 amino acids. The specific fragment sequence of P. infestans shared homology of 99.34% with AVR3a gene sequence. The alignments of amino acids showed that there were three amino acids at position 19, 80 and 103 that were different from that of AVR3a. This seemed to be the reason for the variation in virulence of AVR3a in different isolates of P. infestans.

关 键 词：马铃薯 致病疫霉菌 无毒基因AVR3a 毒性 基因克隆 序列分析 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]