HPLC测定VC银翘片中连翘苷的含量  

Determination of Forsythin in VC Yinqiaopian by HPLC

作　　者：王芳[1] 赵子剑[2] 黄智海[3] 

机构地区：[1]湖南益阳市中心医院,413000 [2]怀化学院,418008 [3]益阳市人民医院,413001

出　　处：《光明中医》2008年第11期1671-1672,共2页GUANGMING JOURNAL OF CHINESE MEDICINE

摘　　要：目的:建立VC银翘片中连翘苷的含量测定方法。方法:采用高效液相色谱法测定,用C18柱(4.0mm×250mm,5μm),以乙腈∶水(25∶75)为流动相,检测波长277nm。结果:连翘苷的线性范围为0.4μg—3.0μg;相关系数r=0.9999连翘苷平均回收率为99.28%,RSD=2.81%(n=5);结论:所用方法测定样品灵敏度高,重复性好,能有效地控制VC银翘片的质量。Objective：To establish method for the determination of Forsythin in VC Yinqiaopian; Methods：The separation was carried out on C 18（3.9 ×150mm,5μm）eolumn; The mobile phase was composed of aeetonitrile-H2O（25：75）, Detection wavelength was 277nm;Results：The linear range of Paeoniflorin was 0. 4μg-3.0μg （ r = 0.9999） ; Average recovery was 99.28% （ RSD = 2.81% ; n = 5）. Conclusion： This method was accurate, rapid.reproducible and suitable for the quality control of this preparation.

关 键 词：VC银翘片 连翘苷 HPLC 

分 类 号：R286[医药卫生—中药学]