纳米四氧化三铁双歧杆菌LTA对实验性胃癌增殖及凋亡的影响  

Effect of Nano-sized-Fe_3O_4-Lipoteichoic acid on proliferation and apoptosis in experimental gastric cancer

作　　者：侯任穷[1,2] 李玉涛[1,2] 张宇[1,2] 刘宁[1,2] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室 [2]食品学院,黑龙江哈尔滨150030

出　　处：《中国微生态学杂志》2008年第5期428-430,共3页Chinese Journal of Microecology

基　　金：国家自然科学基金(30371055)

摘　　要：目的观察纳米四氧化三铁(Fe3O4)双歧杆菌脂磷壁酸(lipoteichoicacid,LTA)对实验性胃癌增殖细胞核抗原(PCNA)和bcl-2、bax蛋白表达的影响。方法用胃癌BGC-823细胞裸鼠皮下移植瘤模型,观察不同浓度纳米Fe3O4双歧杆菌LTA组与LTA组及环磷酰胺组移植瘤的生长情况,免疫组化法检测bcl-2和bax及增殖细胞核抗原(PCNA)的表达。结果纳米Fe3O4双歧杆菌LTA各剂量组胃癌移植瘤PCNA阳性细胞面密度以及bcl-2阳性细胞面密度均显著低于肿瘤对照组(P<0.01),而bax的阳性细胞面密度显著高于肿瘤阴性对照组(P<0.01)。结论纳米四氧化三铁LTA能明显降低人胃癌移植瘤的增殖活性及增强bax的蛋白表达,同时使其bcl-2基因蛋白的表达下调。Objective To explore the effect of Nano-sized-Fe3O2-Lipoteichoic acid from Bifidobacterium on the expression of proliferating cell nuclear antigen（PCNA） ,bcl-2 and bax in experimental gastric cancer in vivo. Methods Nude miees subcutaneously transplanted with BGC-823 human gastric cancer cells were administered with different doses of Nano- sized-Fe3O4-LTA, LTA and CP by intraperitoneal injection. The immunohistoehemical staining was used to detect the expression of bcl-2, bax and PCNA in the transplanted tumor. Results The expression rates of bcl-2 and PCNA were significantly lowered down in Nano-sized-Fe3O4-LTA groups compared with the NS group （P 〈 0.01 ） , while the expression rate of bax was significantly higher in Nano-sized-Fe3O4-LTA compared with the NS group （ P 〈 0.01 ）. Conclusion Nano-sized- Fe3O4-LTA can decrease the proliferating activity of tumor cell and the expression of bcl-2 gene while increasing the expression of bax gene.

关 键 词：纳米四氧化三铁 双歧杆菌 脂磷壁酸 胃癌 

分 类 号：R378.992[医药卫生—病原生物学]