鹅副粘病毒WF_(00)G分离株HN蛋白基因的测序与序列分析  

Sequencing and sequencing analysis of HN gene of goose paramyxovirus WF_(00)G strain

作　　者：胡元庆[1] 路振香[1] 张训海[1] 金光明[1] 陈会良[1] 

机构地区：[1]安徽科技学院家禽疫病防控监测安徽省重点实验室,安徽凤阳233100

出　　处：《中国微生态学杂志》2008年第5期469-471,474,共4页Chinese Journal of Microecology

基　　金：安徽科技学院引进人才专项(ZRC200681);安徽省教育厅自然科学基金一般项目(KJ2007B298)资助

摘　　要：用鹅副粘病毒凤阳分离株WF00G做9～10日龄SPF鸡胚的尿囊腔接种，成功增殖了该病毒，采用一步法RT-PCR技术扩增WF00G病毒的HN基因，获得了1条约1．8kb的特异性条带。PCR产物回收纯化后测序。测序结果表明，扩增片段大小为1844bp，含有1个1716bp的开放性阅渎框，编码571个氨基酸。核苷酸同源性分析表明：WF00G株与其他12株鹅副牯病毒的同源性为89．9％-99．2％，与国内外其他NDVHN基因的同源性为81．7％～95．7％，其中与国内标准强毒株F48E9的同源性为84．7％，说明WF00G与国内外的传统毒株有较大变异。与Taiwan95株和NL／96株的同源性为94．3％和95．7％，说明WF00G与Taiwan95株和NL／96株亲缘关系较近，具有较高的相似性。The goose paramyxovirus of WF00G strain was grown in 10-day-old SPF embrocated eggs. The HN gene of WF00G was amplified by one step RT-PCR. The sequencing analysis showed that the sequence of the HN gene was 1844 bp and encoding a protein of 571 amino acids. The homology between WFooG and the other goose paramyxovirus strains was 89.9% to 99.2%. The homology between WF00G and the NDV strains isolated inside and outside China was from 81.7% to 95.2%. The WF00G HN gene＇s nueleotide sequence share 84.7% homology with that of the NDV strain F48E9. The ORF of WF00G HN gene shared 94.3% and 95.7% nucleotide homology with that of the NDV strain Taiwan95 and NL/96 respectively. The results indicated the mutation on the HN gene had varied largely, compared with classic NDV.

关 键 词：鹅源副粘病毒 HN基因 序列测定 同源性分析 

分 类 号：S852.659.5[农业科学—基础兽医学] Q503[农业科学—兽医学]