大鼠原位肝移植模型手术并发症的分析  

COMPLICATIONS IN SETTING UP THE MODEL OF ORTHOTOPIC LIVER TRANSPLANTATION IN RAT

作　　者：汪泳[1] 宇汝胜[1] 周晓俊[1] 钱海鑫[1] 

机构地区：[1]苏州大学第一附属医院江苏省临床免疫学重点实验室,苏州215006

出　　处：《肝胆外科杂志》2008年第5期355-357,共3页Journal of Hepatobiliary Surgery

基　　金：江苏省自然科学基金资助项目(BK2007057)

摘　　要：目的分析大鼠原位肝移植模型建立中手术并发症的发生原因,以制订防治措施,提高移植成功率。方法用SD大鼠作为供、受鼠,在Kamada等的袖套法大鼠肝移植模型的基础上改进,施行原位肝移植70例,对预实验中并发症的发生情况进行统计,统计在实验中的并发症发生率,并进行对比分析。结果预实验30只大鼠并发症发生率为70%,显著高于正式实验(12.5%)(P<0.05)。结论实验中发现并及时总结并发症的发生情况,通过改进手术方法和技巧,可显著提高正式实验的手术成功率及大鼠存活率。Objective To summarize complications in setting up the model of orthotopic liver transplantation in rat, analyse the causes and take setps to increase the successful rate. Methods 70 SD rats were used to be donors and recipients respectively, according to modified operational method for orthotopic liver transplantation with two-cuff technique to set up the model. We count the rate of the complications during experiments,and analysed the date and compared with these in experiments. Results 30 preliminary experi- ments were done with a failure rate 70% ,it was more seious than the formal experiments （ 12. 5% ） （P 〈 0. 05 ）. Conclusion We de- tected the probability of complications timely during the preliminary experiments, and took steps by amending technique, enhancing skill and dealing with the changes of the pathophysiology, the success rate was improved greatly in the formal experiments.

关 键 词：原位肝移植 大鼠并发症 分析预防 

分 类 号：R575[医药卫生—消化系统]