脑微血管内皮细胞条件培养液对体外培养正常神经元的影响  

The Effect of Cerebral Microvascular Endothelial Cells Conditioned Medium on Cultured Neurons

作　　者：陈会丛[1] 李澎涛[1] 潘彦舒[1] 华茜[1] 杜欢[1] 王筠[1] 刘元[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《世界科学技术-中医药现代化》2008年第4期43-47,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家"973"课题(2005CB523311):通络方药对脑缺血损伤的脑组织保护作用的微血管机制研究;负责人:李澎涛;国家自然科学基金课题(30572284):从络脉论治脑神病的生物学基础研究;负责人:李澎涛;国家"十一五"支撑计划(2006BAI08B05-04):基于脑微血管内皮细胞功能物质发现的脑病药物筛选与效应分析新技术;负责人:李澎涛。

摘　　要：目的:探讨体外模拟脑缺血再灌注损伤条件下及通络救脑注射液干预下脑微血管内皮细胞条件培养液(CMECs-CM)对体外正常培养神经元的影响。方法:先分别进行大鼠大脑微血管内皮细胞和神经元的分离纯化培养及建立体外模拟脑缺血再灌注损伤模型,然后用收集的CMECs-CM 1:1和1:5的浓度作用于体外培养的神经元,MTT法测定神经元的活性、存活率,western-blot检测NF-κB的表达。结果:脑微血管内皮细胞条件培养液可影响神经元生存活性,应用中药通络救脑注射液作用于脑微血管内皮细胞后收集的条件培养液可明显提高神经元的生存活性,可以提高神经元中NF-κB的表达。This paper investigates the effect of cerebral microvascular endothelial cells conditioned medium （CMECs-CM） on cultured neurons. In the study, four types of brain microvascular endothelial cells of the conditioned medium （N-CM, NT-CM, Rp-CM, RpT-CM） were collected, before being added to the cultured cortical neurons respectively. MTT was used to determine the activity of neurons, and calculate the survival rate of neurons. Meanwhile, western-blotting detection was employed to determine the NF-κB expression. It is found that NT-CM can significantly increase the activity of neurons, and raise the survival rate, while Rp-CM significantly reduces the activity of neurons. Comparing with the Rp-CM group, RpT-CM can significantly inhibit the decline of neurons activities, and improve the survival rate of neurons. Cerebral microvascular endothelial ceils conditioned medium （CMECs-CM）, especially treated with TONGLUOJIUNAO injection （TLJN）, may increase the expression of NF-κB on neurons. It is believed that brain microvascular endothelial cells conditioned medium can affect neural activities and functions. Chinese medicine may play a role of protecting the brain functions by affecting the cerebral microvascular endothelial cells.

关 键 词：脑微血管内皮细胞 神经元 脑缺血再灌注 条件培养液 

分 类 号：R743[医药卫生—神经病学与精神病学] R392[医药卫生—临床医学]