原发性胆汁性肝硬化患者抗线粒体抗体亚型检测的临床意义  

Clinical Significance of Detection of Subtypes of Anti-mitochondrial Antibody in Patient with Primary Biliary Cirrhosis

作　　者：冯雪[1] 李永哲[1] 张洋[1] 包书萌[1] 佟大伟[1] 张蜀澜[1] 胡朝军[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院检验科,北京100730

出　　处：《标记免疫分析与临床》2008年第5期265-267,277,共4页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金资助项目(No.30471617;No.30640084)

摘　　要：为探讨原发性胆汁性肝硬化(PBC)患者血清抗线粒体抗体(AMA)亚型抗M2、M4、M9抗体的临床意义,采用间接免疫荧光法检测96例PBC患者血清AMA水平,采用酶免疫斑点法检测AMA-M2、M4、M9抗体水平,应用全自动生化分析仪检测ALT等生化指标。结果表明96例PBC患者中,荧光法AMA阳性率为84.4%,抗M2抗体为81.3%;斑点法抗M2抗体阳性率为72.9%,抗M4抗体为44.8%,抗M9抗体为18.8%,抗M2和M4抗体同时阳性阳性率为43.8%,抗M2和M9抗体同时阳性阳性率为16.7%,抗M2、M4和M9抗体同时阳性阳性率为13.5%,抗M4抗体阳性的PBC患者ALT、AST和IgM水平明显高于抗M4抗体阴性(P<0.05),抗M9抗体阳性的PBC患者ALT、AST和IgG水平明显低于抗M9抗体阴性(P<0.05)。AMA-M2抗体的检测对PBC患者有诊断意义,抗M4抗体和抗M9抗体的检测对于PBC患者的病情判断有意义。To study the clinical sign M2,anti-M4 and anti-M9 in patients ificance of anti-mitochondrial antibody＇ s （AMA） subtypes antiwith primary biliary cirrhosis （PBC） , the serum levels AMA in 96 patients with PBC were detected by indirect immunofluoreseence and anti-M2, anti-M4 and anti-M9 levels by immunodotting, the ALT levels by automatic biochemistry analysator. The results showed that the positive ratio of AMA was 84.4% , the positive ratio of anti-M2 was 81.3% by indirect immunofluorescence. By immunodotting method the positive ratio of anti-M2 was 72.9% , the positive ratio of anti -M4 was 44.8% , the positive ratio of anti-M9 was 18.8% , the positive ratio of both anti-M2 and anti -M4 was 43.8% , the positive ratio of both anti-M2 and anti-M9 was 16.7% , and the positive ratio of anti-M2, anti-M4 and anti-M9 at the same time was 13.5%. The levels of the ALT, AST, IgM in patients with anti-M4 positive were higher than these with the anti-M4 negative （ P 〈 0.05 ）. The levels of the ALT, AST and IgG in patients with anti-M9 positive were lower than these with in anti-M9 negative （ P 〈 0.05 ）. The detection of anti-M2 antibody levels has clinical significance in the diagnosis of PBC. The detection of anti-M4 and anti-M9 levels have a important role to judge the pathogenetic conditions in patients with primary biliary cirrhosis.

关 键 词：原发性胆汁性肝硬化 抗线粒体抗体 酶免疫斑点法 

分 类 号：R575.2[医药卫生—消化系统]