^(18)F-FDG摄取率和其它指标评价热化疗对MCF-7细胞的杀伤作用的比较  

Evaluation of Killing Effect on MCF-7 Cell by Thermochemotherapy Using the Uptake Rate of ^(18)F-FDG

作　　者：张晨鹏[1] 孙晓光[1] 黄钢[1] 刘平安[1] 

机构地区：[1]上海交通大学医学院附属仁济医院核医学科临床核医学研究所,上海200001

出　　处：《标记免疫分析与临床》2008年第5期288-291,共4页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金资助项目(No.50436030);上海市科委重点基金资助项目(04JC14044);上海市教委重点发展基金项目(No.04DA03)

摘　　要：探讨比较18F-FDG摄取率和其它指标在评价热化疗对MCF-7细胞杀伤效果中的作用。采用MTT法检测细胞存活率,流式细胞仪测定细胞凋亡,透射电镜观察细胞超微结构等常规方法观察对MCF-7细胞进行热化疗处理后的细胞变化情况,检测热化疗后细胞对18F-FDG摄取的变化以及细胞培养液中乳酸脱氢酶和肿瘤标志物的变化,评价热化疗疗效结果。结果显示,热化疗能够明显抑制MCF-7细胞的增殖,且诱导MCF-7细胞的凋亡。电镜显示,相比正常对照组,热化疗组的核浓缩,凋亡小体明显增多,细胞空泡形成,细胞器裂解明显。热化疗24h后MCF-7细胞乳酸脱氢酶酶含量变化不明显,葡萄糖含量增多,对18F-FDG摄取百分率明显降低。热化疗影响乳腺癌细胞的葡萄糖代谢,18F-FDG摄取率比乳酸脱氢酶及其它指标能更早反映热化疗的疗效。To explore the killing effect on MCF-7 cell by thermochemotherapy using ^18F-FDG and other indices. In order to observe changes of MCF-7 cell after treatment by thermochemotherapy, the cell survival rate was detected by MTr. The cell apoptosis was checked by flow cytometry and cell ultra structure was observed by transmission electron microscope. The uptake rate of ^18F-FDG and the content of lactate dehydrogenase and tumor markers in cell culture medium were employed to assess therapeutic effect of thermochemotherapy. The results showed that thermochemotherapy significantly inhibited the proliferation of MCF-7 cell and induced cell apoptosis. The results of transmission electron microscope demonstrated that the cells treated with thermochemotherapy has a condensation of nuclear chromatin, apoptotic body formation, cytoplasmic vacuolization and organelle loss compare with normal control cells. The content of lactate dehydrogenase in cell culture medium did not change significantly after 24 h thermochemotherapy, while the content of glucose was increase and resulted in a decrease for the uptake rate of ^18F-FDG. Thermochemotherapy has a great influence on glucose metabolism of ceils. The uptake rate of ^18F-FDG in cell culture medium could be a better index than the content of lactate dehydrogenase and other indices in the evaluation of therapeutic effect of thermochemotherapy.

关 键 词：^18F-FDG 乳酸脱氢酶 热化疗 葡萄糖 

分 类 号：R737.9[医药卫生—肿瘤]