PC-1蛋白单克隆抗体制备和鉴定  

Preparation and Identification of Monoclonal Antibody Against PC-1 Protein

作　　者：高雪松[1,2] 张慧[1] 周立权[3] 孙玉龙[1] 梁瑞霞[1] 陈伟[1] 刘萃龙[2] 周建光[1] 

机构地区：[1]解放军军事医学科学院生物工程研究所,北京100850 [2]解放军海军总医院泌尿外科,北京100037 [3]解放军总医院泌尿外科,北京100853

出　　处：《标记免疫分析与临床》2008年第5期303-305,338,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：为获得高效价PC-1蛋白的鼠单克隆抗体(mAb),用于PC-1功能的实验,采用纯化的PC-1蛋白与GST的融合蛋白(GST-PC-1)为抗原,免疫小鼠和骨髓瘤细胞进行细胞融合,用直接ELISA法进行克隆化筛选,mAb的染色体分析及亚类鉴定及初步应用。结果表明细胞融合和ELISA筛选后,获得2株抗鼠PC-1蛋白mAb,两株PC-1 mAb效价分别为1∶25600,1∶13600,抗体亚型为IgG1,Western blot证实其特异性识别PC-1蛋白。该mAb可以用于测定PC-1蛋白。To prepare monoelonal antibody （mAb） against PC-1 protein used in research of PC-1 protein, two hybridoma cell lines secreting mAb against PC-1 protein were produced by fusing mouse myeloma cells with spleen cells from BALB/c immunized with the fusion protein and GST-PC-1. The infused cells were screened for anti-protein antibody by ELISA. The results showed that the ELISA titers of two mAbs were 1：25600 and 1：13600, respectively and the mAbs were fallen into type IgG1. The mAbs reacted strongly and specifically to protein of PC-1 by Western blot analysis. The prepared mAb may be used to detect protein of PC-1.

关 键 词：抗原 PC-1蛋白 前列腺癌 单克隆抗体 免疫 

分 类 号：R392[医药卫生—免疫学]