N-乙酰氨基苯磺酰氟用于柱前衍生氨基酸的毛细管胶束电动色谱分离  

Separation of amino acids by micellar capillary electrokinetic chromatography using N-acetamidobenzenesulfonyl fluoride as pre-column derivative reagent

作　　者：葛佳璐[1] 屠春燕[1] 赵珺[1] 唐美华[1] 吴燕娇[1] 韦萍[1] 李寿椿 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009 [2]南京中意化工技术开发研究所,南京210037

出　　处：《生物加工过程》2008年第5期68-72,共5页Chinese Journal of Bioprocess Engineering

摘　　要：采用一种新型标记试剂N-乙酰氨基苯磺酰氟(PAABS-F)作为柱前衍生试剂,建立了用毛细管胶束电动色谱分离16种常见氨基酸的方法。以硼砂-三(羟甲基)氨基甲烷(Tris)二元缓冲体系为背景电解液,考察了缓冲液的pH和离子强度、表面活性剂十二烷基硫酸钠(SDS)添加量、有机改性剂种类及分离电压等条件对分离效果的影响,实验结果表明:采用40 mmol/L硼砂-Tris(摩尔比1∶1)缓冲液(pH 9.3)、添加140 mmol/L的SDS、体积分数5%甲醇及10 mmol/L三乙胺作为分离体系,可对16种PAABS-F氨基酸衍生物进行分离。A method for separating sixteen common amino acids by micellar capillary electrokinetic chromatography is used by using N-acetamidobenzenesulfonyl fluoride as pre-column ultraviolet labeling reagent. Sodium dodecyl sulfate concentrations, organic modifiers and pH in the running buffer consisted of sodium tetraborate-Tris were optimized for fast and efficient separation. Experimental results showed that 40 mmol/L sodium borate-Tris （1： l） , pH 9.3, 140 mmol/L sodium dodecyl sulfate, 5% methanol, and 10 mmol/L tri-ethylamine were the optimal conditions. Under the optimal conditions, sixteen common amino acids were successfully separated.

关 键 词：毛细管胶束电动色谱 氨基酸 对乙酰氨基苯磺酰氟 衍生化 

分 类 号：O657.8[理学—分析化学]