锌指转录因子Slug在早孕小鼠子宫内膜的表达  

Expression of Slug Gene in Endometrium of Early Pregnant Mouse

作　　者：杜芳[1] 杨戎[1] 魏莎莉[1] 马海兰[1] 王庆跃[1] 

机构地区：[1]重庆医科大学生殖生理教研室,重庆400016

出　　处：《生殖与避孕》2008年第10期577-581,597,共6页Reproduction and Contraception

摘　　要：目的:初步探讨锌指转录因子Slug在早孕小鼠胚胎植入过程中的作用。方法:用RT-PCR和免疫组织化学方法分别检测未孕及妊娠1-7d小鼠子宫内膜Slug在mRNA水平和蛋白水平的表达。结果:RT-PCR半定量结果显示,早孕小鼠子宫内膜组织Slug的表达高于未孕小鼠子宫内膜组织,且随着妊娠天数的增加呈现逐渐增加的趋势,到妊娠4d达最高。进一步通过免疫组化也显示小鼠子宫内膜有Slug蛋白表达,且其表达规律与RT-PCR结果基本一致。结论:Slug在妊娠早期子宫内膜持续表达,可能参与了胚泡植入过程。Objective： To explore the expression of Slug in mouse endometrium during early pregnancy. Methods： Reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemistry were used to investigate Slug mRNA and protein expression in mouse endometrium of unpregnancy and pregnancy d 1-7, respectively. Results： RT-PCR and immunohistochemiological analysis showed that the expressions of both Slug mRNA and protein in endometrium of pregnant mice were higher than those of non-pregnant mice, and gradually augmented tendency as days went and reached the highest level at pregnant d 4. Conclusion： Upregulation of Slug expression may play a key role during embryo implantation.

关 键 词：SLUG 子宫内膜 胚胎植入 RT—PCR 免疫组化 

分 类 号：R714.8[医药卫生—妇产科学]