恶性胶质瘤细胞株U251中肿瘤干细胞放射敏感性的体外研究  

Radiosensitivity of brain cancer stem cells from malignant glicoma cell line U251 in vitro

作　　者：王彬[1] 杨辉[1] 黄其林[1] 刘海鹏[1] 吕胜青[1] 

机构地区：[1]第三军医大学附属新桥医院神经外科,重庆400037

出　　处：《中华放射医学与防护杂志》2008年第5期505-508,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的应用^60Coγ射线研究不同生存条件下恶性胶质瘤细胞株U251中肿瘤干细胞的放射敏感性。方法^60Coγ射线分别照射U251中的肿瘤干细胞及从U251中分离、培养的肿瘤干细胞后，采用TUNEL、Annexin—FITC等方法检测细胞凋亡，行裸鼠皮下移植以及应用流式细胞仪检测这两种不同生存条件下的细胞周期进程情况。结果体外单独体外存活的肿瘤干细胞处于活跃的细胞周期进程中，对射线敏感，经^60Coγ射线照射后凋亡细胞明显增多，裸鼠皮下移植后不再成瘤。而在U251细胞中存活的肿瘤下细胞处于G0—G1期，对射线抗拒，裸鼠皮下移植后继续分化成为亲本肿瘤的胶质瘤类型。结论恶性胶质瘤细胞株中的肿瘤干细胞因对射线抗拒而有可能成为胶质瘤经过放射治疗后原位复发的原因。Objective To investigate the radiosensitivity of brain cancer stem cells of different conditions isolated from malignant glioma cell line U251 in vitro. Methods The brain cancer stem cells in U251 or the brain cancer stem cells isolated from U251 were irradiated by ^60Co γ-rays. TUNEL and Annexin-FITC were employed to detect the apoptosis. The brain cancer stem cells were subcutaneously transplanted to nude mouse. Flow cytometry was used to detect cell cycle. Results The brain cancer stem cells isolated from malignant glioma cell line U251 were in active cell cycle and sensitive to ^60 Co γ-rays. Thed apoptotic cells were increased obviously after in＇adiation. After subcutaneously transplanted to unde mouse, there was no tumor appear. However, the brain cancer stern cells existed in U251 were in G0-G0 and resisted to ^60Co γ-rays. They differentiated into the parent glioma type after traqnsplantation. Conclusions The brain cancer stem cells existed in the malignant glioma cell line is resisted to irradiation, and this phenomenon may explain the glioma relapse in situ after radiation therapy.

关 键 词：胶质瘤 肿瘤十细胞 免疫磁珠 放射敏感性 

分 类 号：R739.4[医药卫生—肿瘤]