siRNA对白血病细胞株HEL中EDAG-1基因的干涉作用  

Effect of siRNA Targeting EDAG-1 Gene in Leukemia Cell Line HEL

作　　者：周颖[1] 程勇[2] 高宗侠[1] 肖敏[1] 冯定庆[1] 沈国栋[1] 凌斌[1] 

机构地区：[1]安徽省分子医学重点实验室安徽省立医院分子实验室,合肥230001 [2]安徽省立医院肿瘤放疗科

出　　处：《肿瘤防治研究》2008年第10期688-690,共3页Cancer Research on Prevention and Treatment

基　　金：安徽省教育厅自然科学研究资助项目(2006KJ080C)

摘　　要：目的探讨抑制胚胎发育相关基因-1(EDAG-1)的表达对白血病细胞株生长的影响及其机制。方法收集重组质粒转染包装病毒细胞的上清转染HEL细胞株,经嘌呤霉素筛选稳定抑制EDAG-1基因表达的细胞株,RT-PCR检测EDAG-1基因的抑制情况,运用MTT检测转染细胞的增殖能力,并将稳定转染的细胞株种植到裸鼠皮下检测成瘤情况。结果成功建立了抑制EDAG-1基因表达的HEL细胞株。与HEL/negative相比,HEL/siRNA细胞株增殖速度降低(P<0.05),接种裸鼠皮下的肿瘤生长明显减慢(P<0.05)。结论沉默EDAG-1基因的表达可以抑制白血病细胞株的增殖,提示EDAG-1基因可能是白血病治疗的一个有效靶点。Objective The purpose of this study is to explore the effects of silence of EDAG-1 on the growth of leukemia cell line and its mechanism. Methods HEL was infected by the virus supernatant collected from packing cell line transfected by recombinant retroviral vector, the stable cell lines were selected with puromycin. The reduction of EDAG-1 was inspected with RT-PCR; and proliferation was assayed with MTT. The tumor growth of the null mice was analyzed after injection HEL/siRNA, HEL/ negative into the skin. Results The stable HEL cell lines with a persistent knockdown of EDAG-1 were established. Comparison with HEL/negative, the proliferation of HEL/siRNA was inhibited significantly（P〈0. 05）, the growth of HEL/siRNA implanted tumor slowed down significantly（P〈0. 05）. Conclusion Inhibition of the expression of EDAG-1 can reduce the proliferation of leukemia cell lines, suggesting that EDAG-1 may be an effective target for the treatment of leukemia.

关 键 词：EDAG-1 RNA干扰 白血病 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]