survivin选择性剪接变体在人结直肠癌中的表达及其与临床病理参数的关系  

Correlation between survivin Expression and Clinicopathological Factors in Colorectal Cancers

作　　者：付文荣[1] 张勤[1] 程正江[2] 

机构地区：[1]华中科技大学同济医学院附属襄樊医院病理科,湖北襄樊441021 [2]华中科技大学同济医学院附属协和医院检验科

出　　处：《肿瘤防治研究》2008年第10期719-722,共4页Cancer Research on Prevention and Treatment

摘　　要：目的探讨结直肠癌survivin选择性剪接变体表达及其与临床病理参数之间的关系,揭示其在结直肠癌发生发展中的作用及临床意义。方法RT-PCR结合免疫组织化学技术,对survivin各种剪接变体在结直肠癌和癌旁组织中的表达进行了研究,同时还对survivin剪接变体survivin-2B及survivin-ΔEx3表达和临床病理资料作了相关性分析。结果结直肠癌组织和癌旁组织都表达survivin,并且得到免疫组织化学的证实;survivin-2B主要表达于癌旁组织,而survivin-ΔEx3则主要在癌组织中表达且与肿瘤转移相关。结论survivin不同变体表达之间的平衡关系可能对肿瘤的发生和发展起重要作用。Objective To investigate the expression of survivin variants and their relationship to each other as well as to clinic pathological factors in colorectal cancer. Methods survivin variant specific RT-PCR was developed to analysis their expression in 67 paired cancer and para-cancer tissues, and the expression of the wildtype survivin at the protein level was further verified by immunohistochemistry. Results All the cases tested expressed survivin mRNA, and immunohistochemistry obtained similar finding. The nonantiapoptostic survivin-2B was dominantly expressed in para-cancer tissues, whereas the antiapoptosticsurvivin-△EX3 was more frequently detected in cancer tissues. expression of survivin-△EX3 was significantly associated tumor balance metastasis. Conclusion The mRNA between antiapoptostic with The sur-vivin iso-forms and nonantiapoptostic ones may play an important role in tumor genesis and tumor progression.

关 键 词：SURVIVIN基因 剪接变体 结直肠癌 RT-PCR 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]