核心结合因子α1对牙本质涎磷蛋白基因启动子不同片段启动活性的影响  

Core binding factor α1 regulates the promotive activity of different segments of mouse dentin sialophosphoprotein promoter

作　　者：郭婷[1] 曹罡[1] 余擎[2] 肖明振[2] 赵守亮[2] 陆斌[2] 

机构地区：[1]南京军区南京总医院口腔科,江苏南京210002 [2]第四军医大学口腔医学院,陕西西安710032

出　　处：《医学研究生学报》2008年第10期1014-1017,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30271418);南京军区南京总医院科研基金资助项目(批准号:2006052)

摘　　要：目的:观察核心结合因子α1(cbfα1)对牙本质涎磷蛋白(DSPP)基因启动子不同片段启动活性的影响。方法:选择小鼠成牙本质细胞样细胞MDPC-23为实验细胞,利用含DSPP基因启动子不同片段的真核表达载体,采用报告基因方法观察cbfα1对DSPP基因启动子不同片段启动活性的影响。结果:在MDPC-23细胞中,pcDNA3-cbfα1共转染组相对于pcDNA3共转染组,pGL3-Enhancer-1(-4496～-3499 bp)、pGL3-Enhancer-2(-3519～-2515 bp)、pGL3-Enhancer-2-3、pGL3-Enhancer-95(-95～54 bp)活性增强(P<0.05);pGL3-Enhancer-1-3、pGL3-Enhancer-2.6 K(-2475～53 bp)活性降低(P<0.05)。结论:cbfα1可以调控DSPP基因的表达,cbfα1对DSPP基因启动子不同片段的启动活性有不同的调节作用。Objective： To study the effect of the core binding factor α1（cbfα1） on the promotive activity of different segments of the mouse dentin sialophosphoprotein（DSPP） promoter.Methods： We co-transfected the report vectors of different segments of the mouse DSPP promoter and pcDNA3-cbfαl or pcDNA3 into MDPC-23 cells using the Lipofectamine^TM2 000,measured the cells for luciferase activity with the dual luciferase reporter assay system and analyzed the results by the SPSS10.0 Software for Windows.Results： Compared with the pcDNA3 co-transfected group,the promotive activity of different segments of the mouse DSPP promoter and pcDNA3-cbfαl in MDPC-23 cells was obviously increased in pGL3-Enhancer-1（-4 496^-3 499 bp）,pGL3-Enhancer-2（-3 519^-2 515 bp）,pGL3-Enhancer-2-3 and pGL3-Enhancer-95（-95~54 bp）（P〈0.05）,but markedly decreased in pGL3-Enhancer-1-3 and pGL3-Enhancer-2.6K（-2 475~53 bp）（P〈0.05）.Conclusion： Cbfα1 can regulate the expression of DSPP as well as the promotive activity of different segments of the DSPP promoter.

关 键 词：核心结合因子Α1 牙本质涎磷蛋白 瞬时转染 报告基因 转录调控 

分 类 号：Q75[生物学—分子生物学]