FMNL2在大肠癌细胞系中的表达及意义  

Expression of FMNL2 and its relation to the metastatic potential of human colorectal cancer cells

作　　者：朱曦龄[1] 梁莉[1] 丁彦青[1] 

机构地区：[1]南方医科大学病理学教研室,广东广州510515

出　　处：《南方医科大学学报》2008年第10期1775-1778,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30400206)

摘　　要：目的检测FMNL2在大肠癌细胞系中的表达情况,初步探讨其与大肠癌侵袭转移的关系。方法(1)用Real time-RTPCR法和免疫组化法分析FMNL2 mRNA在6种大肠癌细胞系的表达情况;(2)通过shRNA质粒载体瞬时干扰大肠癌细胞株中FMNL2的表达,Boyden小室法比较干扰前后细胞侵袭能力的变化情况;(3)用Boyden小室法比较6种大肠癌细胞系的侵袭转移能力。结果(1)FMNL2在大肠癌转移灶来源的细胞系中的表达高于大肠癌原发灶来源的细胞系;(2)大肠癌细胞株中FMNL2的表达下调后,细胞的侵袭转移能力明显下降;(3)Boyden小室的结果显示大肠癌转移灶来源的细胞系的侵袭,转移能力高于大肠癌原发灶来源的细胞系。结论FMNL2的高表达可能与大肠癌的侵袭转移有一定的相关性。Objective To explore the association of FMNL2 expression with the metastatic potential ofcolorectal cancer cells. Methods FMNL2 mRNA and protein expressions in 6 human colorectal cancer cell lines were detected by real-time RT-PCR and immunohistochemical method, respectively, and analyzed for their correlations to the in vitro invasiveness of the cell lines evaluated by Boyden assay. In SW620 and SW480/M5 cell lines, the expression of FMNL2 was repressed by FMNL2 short hairpin RNA （shRNA）, and the changes in the invasiveness of the cells were observed. Results FMNL2 was highly expressed in SW480/M5, LoVo and SW620 cells derived from metastatic colorectal cancers in comparison with that in LS174T, SW480 and HT29 cells, which were derived from primary colorectal cancers. In vitro analysis of the cell invasiveness demonstrated that SW480/M5, LoVo and SW620 cells had higher invasiveness than LS174T, SW480 and HT29 in vitro. In SW480/M5 and SW620 cells, transfection with FMNL2 shRNA resulted in significantly lowered cell invasiveness. Conclusion FMNL2 may play an important role in the invasion and metastasis ofcolorectal cancer.

关 键 词：大肠癌 FMNL2 转移 侵袭 

分 类 号：R735.34[医药卫生—肿瘤]