耐药乳腺癌细胞株MCF-7/ADR中microRNA的分析  

Analysis of microRNA in drug-resistant breast cancer cell line MCF-7/ADR

作　　者：崔秀英[1] 郭运杰[2] 姚和瑞[2] 

机构地区：[1]中山大学附属第二医院乳腺外科,广东广州510120 [2]中山大学附属第二医院肿瘤科,广东广州510120

出　　处：《南方医科大学学报》2008年第10期1813-1815,共3页Journal of Southern Medical University

基　　金：广东省自然科学基金(7001592);广东省医学科研基金(A2005226);广东省科技计划(2006B36002010)

摘　　要：目的本研究通检测乳腺癌细胞株MCF-7及其阿霉素耐药株MCF-7/ADR的microRNA的表达差异,探讨microRNA与乳腺癌化疗耐药的关系。方法MTT法检测MCF-7/ADR相对于其亲本细胞MCF-7的耐药性和耐药倍数;microRNA芯片和RT-PCR的方法比较阿霉素耐药细胞株MCF-7/ADR与其对应的MCF-7乳腺癌细胞株microRNA表达差异。结果乳腺癌的阿霉素耐药株MCF-7/ADR相对于其亲本细胞系MCF-7对阿霉素的耐药倍数为33.2倍。microRNA芯片的结果显示耐药株MCF-7/ADR与亲本细胞系MCF-7比较有16个microRNA高表达,20个microRNA低表达;RT-PCR的结果进一步证实mir-221、mir222、mir-130a、mir-155在MCF-7/ADR中显著上调,而mir200a、mir-200b、mir-200c、mir-421在MCF-7/ADR中显著下调。结论MCF-7/ADR与MCF-7的microRNA的表达存在着差异,提示microRNA参与乳腺癌化疗耐药,为进一步研究microRNA在乳腺癌耐药机制中的作用打下基础。Objective To analyze the difference in microRNAs expression between MCF-7 and MCF-7/ADR cells and explore the association between microRNA and drug resistance of breast cancer. Methods The drug resistance of MCF-7/ADR cells was evaluated using MTT assay and flow cytometry. Microarray technique and RT-PCR were used to analyze the differential expressions of the microRNA between MCF-7 and MCF-7/ADR cells. Results The drug resistance index of MCF-7/ADR cells relative to the parental MCF-7 cells was 33.2. The percentages of the side population in MCF-7/ADR and MCF-7 cells were （9.50 ±0.9）% and （0.85 ±0.2）%, respectively. Microarray analysis of MCF-7 to MCF-7/ADR cells identified 36 differentially expressed genes, including 16 up-regulated and 20 down-regulated genes in MCF-7/ADR cells. RT-PCR identified 14 microRNAs that were differentially expressed between MCF-7 and MCF-7/ADR cells, including 7 up-regulated and 7 down-regulated ones in MCF-7/ADR cells. Of these differentially expressed microRNAs, mir-221, mir222, mir-130a, and mir-155 showed significantly increased expression, and mir200a, mir-200b, mir-200c, and mir-421 showed significantly lowered expression in MCF-7/ADR cells as indicated by the results of microarray analysis and RT-PCR. Conclusion MCF-7/ADR cells show a different microRNA expression profile fi＇om its parental MCF-7 cells, suggesting the involvement of microRNAs in tunaor cell drug resistance. This finding provides a experimental basis for further study of mechanism underlying the drug resistance of breast cancer.

关 键 词：乳腺癌 多药耐药 MICRORNA 

分 类 号：R737.9[医药卫生—肿瘤]