肝细胞癌组织SPARC表达及其临床意义  

The expression of SPARC in hepatocellar carcinoma and its significance

作　　者：陈科济[1] 王义[1] 孙延富[1] 

机构地区：[1]第二军医大学附属东方肝胆外科医院肝外二科,上海200438

出　　处：《中国癌症杂志》2008年第10期744-748,共5页China Oncology

摘　　要：背景与目的:最近的研究发现半胱氨酸的酸性蛋白(SPARC)与肿瘤的发生、进展以及肿瘤的侵袭和转移关系密切,SPARC在黑色索瘤、胶质瘤、脑膜瘤、膀胱癌、肺癌和前列腺癌等多数肿瘤中均有高表达,而且与某些肿瘤的临床分期和预后相关。本研究观察SPARC在人肝细胞癌(hepatocellar carcinoma,HCC)组织中的表达情况。方法:选择62例有完整随访资料的HCC及相应癌旁组织标本、30例正常肝组织标本,用RT-PCR方法检测SPARC mRNA的表达;应用免疫组化染色法检测SPARC在HCC、癌旁组织和正常肝组织的表达。结果:SPARC mRNA在HCC组织呈高表达(14.0±3.6),癌旁组织次之(6.8±1.8),正常肝组织低表达(2.7±0.9),3组间差异均有显著性(P=0.000)。SPARC免疫组化显示62例HCC组织中54例SPARC表达阳性,正常肝组织中仅4例SPARC表达阳性,两者间差异有显著性(P=0.000);SPARC的免疫组化评分在肝癌组为21.5±4.8;癌旁组为11.3±3.6;正常肝组织为5.7±1.8,3组间差异亦有显著性(P=0.000)。SPARC的表达水平随着Enmondson病理分级明显增加;在Ⅰ级和Ⅱ级之间差异有显著性(P=0.029);Ⅱ级和Ⅲ/Ⅳ级间具有显著性(P=0.008)。SPARC表达在出现淋巴结转移的HCC中占26/27(96.3%),显著高于无淋巴结转移者23/35(65.7%)。结论:SPARC表达与肝癌的分化程度,淋巴结转移能力相关,通过检测SPARC表达将对肝癌临床评估有一定意义。Background and purpose：Recent researches have shown that Secreted Protien Acidic and Rich in Cysteine （SPARC） was closely related to tumor genesis, tumor progression and tumor metastasis. SPARC was highly expressed in malignant melanoma, glioma, meningioma, bladder cancer, lung cancer and prostate cancer, etc. In this study we investigated SPARC expression in hepatocellar carcinoma （HCC） and its signifi cance. Methods：RT-PCR was used to detect SPARC mRNA expression in cancer tissue samples and their adjacent liver tissue samples from 62 patients with hepatocellar carcinoma and 30 normal liver tissue samples, respectively. And the differential protein expression of SPARC between these groups was analyzed by immunohistochemistry （IHC）. Results：SPARC mRNA was highly expressed in HCC（14.0±3.6） and in the adjacent liver tissue （6.8±1.8）; compared with low expression of 2.7±0.9 in normal liver tissue, there were signifi cant differences among the three groups （p=0.000）. SPARC positively stained was found in 54 of 62 patients with HCC and 4 of 30 normal liver tissue, there was significant difference between these two groups （P=0.000）. SPARC immunohistochemical score was 21.5±4.8 in the carcinoma group; 11.3±3.6 in paracarcinoma group and 5.7±1.8 in the normal group, there were also significant differences among the three groups （P=0.000）. The expression of SPARC protein was significantly upregulated with the progress of Enmondson pathological classification. There was obviously differences between Ⅰvs Ⅱ（P=0.029）, and Ⅱ vs Ⅲ Ⅳ（P=0.008）. There was more SPARC expression in the patients with metastasis of HCC （26/27, 96.3%） than that without metastasis（23/35, 65.7%）（P=0.004）. Conclusion：SPARC mRNA expression and its protein were related to HCC histological differentiation and metastatic lymph node; SPARC is helpful to clinical evaluation of HCC.

关 键 词：肝细胞癌 半胱氨酸的酸性蛋白 基因表达 组织分化 

分 类 号：R735.7[医药卫生—肿瘤]