基因工程菌漆酶用于提取黄芪总皂甙的研究  

STUDY ON THE EXTRACTION OF ASTRAGALUS SAPONINS WITH RECOMBINANT LACCASE FROM GENE ENGINEERED STRAIN

作　　者：郭梅[1] 路福平[2] 刘敏尧 蒲军[2] 李拖平[1] 梁鹏[1] 

机构地区：[1]天津农学院食品科学系,天津300384 [2]天津科技大学生物工程学院工业微生物重点实验室,天津300222 [3]天津诺奥科技发展有限公司,天津300384

出　　处：《食品研究与开发》2008年第11期75-78,共4页Food Research and Development

基　　金：天津市自然科学基金重点项目(07JCZDJC03100)

摘　　要：利用基因工程菌甲醇毕赤酵母(PMAD16/pMETαA-Lcc1)培养产生的重组漆酶,采用正交试验,对水浸提取条件、漆酶酶解条件进行了研究。结果表明:黄芪总皂甙最佳提取工艺为:先用重组漆酶酶解,1g黄芪加入3.0mL纯化的重组漆酶(453U/mL),在40℃、pH4.0的条件下酶解1.5h,然后再水浸提取黄芪总皂甙。经过重组漆酶酶解处理后黄芪总皂甙提取率比未经酶处理的提取率提高了65.6%。The impacts of the recombinant laccase from gene engineered strain Pichia methanolica on the optimum conditions of enzymolysis process by orthogonal design were studied. The results show the optimum condition of astragalus saponins extraction technique is as following： First, laccase treatment. 1 g astragalus plus into 3.0 mL （453 U/mL） purified recombinant laccase, temperature at 40 ℃, pH value at 4.0 and active time at 1.5 h. Then, water-extracting. Compared with water-extracting, the enzyme-based extraction rate of astragalus saponins is increased by 65.6 %.

关 键 词：基因工程菌 重组漆酶 黄芪总皂甙 正交试验 

分 类 号：TQ461[化学工程—制药化工]