粒细胞集落刺激因子对高脂血症大鼠颈总动脉球囊损伤后再内皮化的影响  

Effect of G-CSF on Endothelial Regeneration after Balloon Injury in the Common Carotid Artery of Hyperlipemia Rats

作　　者：尹小燕[1] 朱健[1] 周龙女[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院急诊科,200011

出　　处：《微循环学杂志》2008年第4期1-3,7,F0002,共5页Chinese Journal of Microcirculation

摘　　要：目的:探讨粒细胞集落刺激因子(G-CSF)对高脂血症大鼠颈总动脉球囊损伤后再内皮化的影响。方法:Wistar大鼠66只随机分为二组:普通饮食组(普食组,n=6)和高脂血症组(高脂组,n=60),高脂组又随机分为:G-CSF+高脂组(n=30)、生理盐水+高脂组(NS+高脂组,n=30)。高脂饮食后1个月血生化指标检测确认高脂血症形成,普食组和G-CSF+高脂组分别皮下注射G-CSF共6天,NS+高脂组皮下注射NS共6天,然后行左颈总动脉球囊拉伤术。采用光镜及扫描电镜观察损伤血管内皮再生情况,采用流式细胞仪观察外周血内皮祖细胞(EPCs)的数量。结果:G-CSF干预后与NS干预相比损伤血管再内皮化加速,损伤后第16天G-CSF干预组内皮再生面积占血管腔面积的百分比较NS组明显增加(P<0.01),血EPCs数量明显增多。结论:G-CSF可以加速高脂血症大鼠损伤血管的再内皮化,其机制可能与外周血EPCs数量的增加参与内皮细胞再生和内膜的修复过程有关。Objective:To observe the effect of G-CSF on the endothelium regeneration after balloon injury in the hyperlipemia rats.Method:A total of 66 male Wistar rats were divided into two groups:normal diet group(control group n=6)and fat diet group(high fat diet group,n=60),high fat diet group include NS+high fat diet group(n=30)and G-CSF+high fat diet group(n=30),high fat diet group was fed with fat diet for 1 month.Before the angioplasty G-CSF or NS was injected subcutaneous,in the same time peripheral blood was collected for measuring the number of endothelial pragenitor cells(EPCs)with flow cytometry.After six days' injection the balloon catheter injury was performed on the left common carotid artery,then morphologic studies were made with light microscopy and scanning electron microscopy to observe the endothelial regeneration.Results:G-CSF significantly accelerated the reendothelialization of injured arteries.The area of reendothelialization in G-CSF group was significantly increased compared with NS group at 16 d after injury(P<0.01).At the same time G-CSF increased the number of EPCs in peripheral blood.Conclusion:G-GSF has a beneficial effect on the reendothelialization of hyperlipemia artery induced by the balloon injury,the mechanism may be related to its mobilization of the EPCs.

关 键 词：粒细胞集落刺激因子 再内皮化 动脉球囊损伤 高脂血症大鼠 WISTAR大鼠 血液生长因子 粒系祖细胞 血管内皮细胞 

分 类 号：R730.53[医药卫生—肿瘤] R543.6[医药卫生—临床医学]