高效液相色谱法测定发酵液中的武夷菌素  

HPLC Determination of Wuyiencin in Fermentation Broth

作　　者：王立东[1,2] 张克诚[1] 石义萍[1] 崔增杰[1] 张思林 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100094 [2]绵阳无公害农产品监测中心,四川绵阳621000

出　　处：《农药》2008年第11期816-817,共2页Agrochemicals

基　　金：科技部农业科技成果转化项目(05EFN216900354)

摘　　要：建立应用高效液相色谱法测定发酵液中武夷菌素含量方法。色谱柱ZORBAX SB-AQ(4.6mm×250mm i.d.,5μm);柱温25℃;流动相为1.4g/L三氯乙酸水溶液,流速1mL/min;检测波长254nm。将色谱峰面积与武夷菌素质量浓度进行线性回归,表明在0.2～1.6g/L质量浓度范围内线性关系良好,变异系数为0.43%～2.08%,回收率为96.59%～100.57%。A high performance liquid chromatography （HPLC） method for the determination of wuyiencin of an agricultural antibiotic in fermentation broth was developed. Analytical conditions involved use of a SB-AQ column （4.6 mm × 250 mm i.d., 5 μm） with temperature of 25 ℃. The mobile phase was 1.4 g/L trichloroacetic acid,flow rate was 1 mL/min and detection wavelength was 254 nm. It also showed a good linearity within the range of 0.2-1.6 g/L. The recovery rate was 96.59-100.57% and the relative standard deviation was 0.43-2.08%.

关 键 词：高效液相色谱 武夷菌素 发酵液 三氯乙酸 测定 

分 类 号：TQ450.7[化学工程—农药化工]