FIL-6与TNF—α、TGF—β在EAE大鼠表达的相关性分析  

Correlation Analysis of the Expressions of IL-6 , TNF-αand TGF-β in Experimental autoimmune Encephalomyelitis in Rats

作　　者：段大志[1,2] 刘勇[2] 梁丽云[1] 马存根[1] 

机构地区：[1]山西大同大学脑科学研究所,山西大同037008 [2]第三军医大学新桥医院神经内科

出　　处：《中国医师杂志》2008年第10期1314-1317,共4页Journal of Chinese Physician

基　　金：山西省2003年度回国留学人员科研基金资助项目（2003-69）,国家人口和计划生育委员会科研资助项目

摘　　要：目的探索IL-6在实验性自身免疫性脑脊髓炎（EAE）中的作用机制。方法将25只雌性Wistar大鼠随机分为EAE组（n=15）、对照组（n=10）。利用免疫组化染色技术观察IL-6、TNF-α、TGF—β的表达，进行相关性分析。结果IL-6灰度值与症状评分呈负相关（r=-0．953，P〈0．05）、与TNF-α呈正相关（r=0．967，P〈0．05），IL-6与TGF—β呈无相关（r=-0．492，P〉0．05）.TNF—α灰度值与症状评分呈负相关（r=-0．978，P〈0．05），TNF—α与TGF—β无相关（r=-0．502，P〉0．05）。TGF-β灰度值与症状评分无相关（r=0．470，P〉0．05）。结论IL-6参与EAE的过程，可能是一种致炎因子。Objective To explore the mechanism of IL-6 in EAE. Methods 25 female Wistar rats were randomly divided into 2 groups, EAE group（ n = 15） and normal control group（ n = 10）. The expression of IL-6, TNF-αand TGF-β of the two groups rats were observed by immunohistochemistry staining, and then correlation analysis of the expression of IL-6, TNF-αandTGF-β was made. Results There was negative correlation between gray scale of IL-6 and symptom scores（ r = -0. 953, P 〈 0. 05）. IL-6 and TGF-β had no statistical- ly significant correlation （ r = -0. 492, P 〉 0. 05 ）. There was negative correlation between gray scale of TNF-α and symptom scores（ r = -0. 978, P 〈0. 05）, and both of the correlation between TNF-α and TGF-β3 （ r = -0. 502, P 〉0. 05） , TGF-β and scores（ r =0. 470, P 〉 0. 05 ） were not statistically significant. Conclusions IL-6 may participate in EAE as a inflammatory factor.

关 键 词：脑脊髓炎 实验性自身免疫性 白细胞介素6 肿瘤坏死因子 转化生长因子β 

分 类 号：R74[医药卫生—神经病学与精神病学]