茉莉酸甲酯与二氢茉莉酮酸甲酯对悬浮培养的甘草细胞生长和黄酮积累的影响  

Effects of Methyl Jasmonate and Methyl Dihydrojasmonate on the Cell Growth and Flavonoids Accumulation in Cell Suspension Culture of Glycyrrhiza inflata Bat.

作　　者：杨英[1] 郑辉[1] 李赟[1] 季家兴[1] 余龙江[1] 

机构地区：[1]华中科技大学生命科学与技术学院,武汉430074

出　　处：《植物生理学通讯》2008年第5期903-906,共4页Plant Physiology Communications

基　　金：新疆建设兵团新疆昆仑神农股份有限公司提供经费.

摘　　要：建立了稳定的甘草细胞悬浮培养体系,在一个培养周期内,细胞的生长曲线呈"S"型,培养21d的干重、鲜重和黄酮产量都达到最高值。甘草细胞悬浮培养体系中分别添加100μmol·L-1二氢茉莉酮酸甲酯和茉莉酸甲酯时,虽然对细胞生长有一定程度的抑制,但细胞中甘草黄酮产量仍有提高。添加二氢茉莉酮酸甲酯和茉莉酸甲酯的最适时间分别为细胞培养后的第5天和第10天。The effects of methyl jasmonate and methyl dihydrojasmonate on cell growth and flavonoids production in suspension cultures of Glycyrrhiza inflata Batal were investigated. Biomass accumulation and flavonoids production both exhibited ＂S＂ curve in one culture cycle, with the greatest values obtained on day 21. In addition, although the cell growth was restrained to some degree by the methyl dihydrojasmonate and methyl jasmonate treatment with the concentration of 100 μmol·L^-1, the flavonoids accumulation was increased. Moreover, the optimum time for the addition of methyl dihydrojasmonate and methyl jasmonate was the 5th day and the 10th day during the cell culture, respectively.

关 键 词：胀果甘草悬浮细胞 甘草黄酮 茉莉酸甲酯 二氢茉莉酮酸甲酯 

分 类 号：S567.71[农业科学—中草药栽培]