软骨损伤患者关节镜手术及关节腔内注射玻璃酸钠后关节液中基质金属蛋白酶-13表达水平变化的研究  

Variation of MMP-13 in Synovia of Patients with Cartilage Injury after Arthroscopic Operation and Intraarticular Injection of Sodium Hyaluronate

作　　者：班吉鹤[1] 周利武[1] 郭亭[1] 吴俊[1] 唐祖明[2] 赵建宁[1] 

机构地区：[1]南京军区南京总医院骨科,江苏省南京市210002 [2]东南大学生物医学工程系分子与生物电子学国家重点实验室

出　　处：《中国骨与关节损伤杂志》2008年第11期902-905,共4页Chinese Journal of Bone and Joint Injury

基　　金：江苏省自然科学基金资助项目(BK2005085)

摘　　要：目的探讨行关节镜手术并关节腔内注射玻璃酸钠后的软骨损伤患者关节液中基质金属蛋白酶-13(MMP-13)表达水平的变化。方法对42例经Outerbridge软骨损伤评分法分组的软骨损伤患者,应用低密度蛋白质芯片方法检测各组患者行关节镜手术前的关节液及术后1、2、3、4周注射玻璃酸钠后的关节液中MMP-13的荧光强度。结果软骨损伤严重组(Ⅳ度、Ⅲ度)的行关节镜手术及玻璃酸钠治疗后的第2周,关节液内MMP-13表达水平开始显著下降,并持续到第5周。软骨损伤较轻组(0度、Ⅰ度)的关节液中MMP-13表达水平在第4、5周与术前的比较有统计学差异(P<0.05)。在第5周时,软骨损伤严重组(Ⅳ度、Ⅲ度)关节液内MMP-13表达水平高于软骨损伤较轻组(0度、Ⅰ度)。结论经关节镜手术、关节腔内注射玻璃酸钠治疗后软骨损伤严重的患者关节液内MMP-13表达水平下降较软骨损伤轻的更明显,MMP-13表达水平下降的意义对软骨代谢水平评价及临床疗效判定有一定的指导价值。Objective To investigate the variation of MMP- 13 in the synovia of patients with the cartilage injury after arthroscopic operation and intraarticular injection sodium hyaluronate. Methods Forty - two patients with cartilage injury were divided into 5 groups by Outerbridge＇ s score. The fluorescence intensity of the MMP- 13 in the synovia was detected before arthroscopie operation and after postoperative intraarticular injection of sodium hyaluronate at the first, second, third and fourth week. Results At the second week, the MMP - 13 in the synovia of the groups with cartilage serious injury （grade Ⅳ, grade Ⅲ ） began to descend significantly, and lasted at the fifth week; the MMP - 13 in the synovia of the groups with the cartilage slight injury （grade Ⅰ） at the fourth and fifth week were lower than that before operation （P 〈 0.05）, at the fifth week, the MMP- 13 in the synovia of the groups with cartilage serious injury （grade Ⅳ, grade Ⅲ） were higher than the cartilage slight injury （grade 0, grade Ⅰ ） . Conclusion The MMP- 13 expression level in the synovia of the groups with cartilage serious injury descends more significantly than that with the slight cartilage injury. There are some instruction value that the sense of the MMP - 13 expression level descending is used for evaluation of the metabolic level of the cartilage and assessment of the clinical effect.

关 键 词：软骨损伤 蛋白质芯片 基质金属蛋白酶-13 玻璃酸钠 

分 类 号：R575.2[医药卫生—消化系统] R684.3[医药卫生—内科学]