低频超声对阿霉素杀伤人白血病多药耐药细胞K562/A02作用的影响  

Antitumor Effects of Low-frequency Ultrasound Combined with Adriamycin on Human Leukemia Multidrug Resistance Cell Line K562/A02

作　　者：孟庆齐[1,2] 陈宝安[1] 吴巍[3] 邵泽叶[1] 高峰[1] 赵慧慧[1] 

机构地区：[1]东南大学附属中大医院血液科,江苏南京210009 [2]南京医科大学第二附属医院血液科 [3]东南大学公共卫生学院,江苏南京210009

出　　处：《癌症》2008年第11期1182-1185,共4页Chinese Journal of Cancer

基　　金：国家自然科学基金资助项目(No.30070318)~~

摘　　要：背景与目的:有研究表明,超声可增加化疗药物对肿瘤细胞的敏感性,从而抑制细胞增殖。本研究中我们观测不同剂量的低频超声波联合阿霉素(adriamycin,A02)对人白血病耐药细胞株K562/A02的生物学影响,并探讨可能的机制。方法:将对数生长期的K562/A02细胞分为空白对照组、单纯阿霉素组、单纯超声组、阿霉素加超声组。24孔培养板上进行超声照射,台盼蓝拒染法、MTT法检测细胞活性,应用瑞姬氏染色法和流式细胞仪检测细胞凋亡和药物浓度,免疫细胞化学检测细胞膜P-糖蛋白的表达变化。结果:频率20kHz、声强0.25W/cm2、辐射时间60s的超声辐射联合7.5μg/mL阿霉素可诱导细胞凋亡,当超声频率20kHz,声强0.5W/cm2时,对细胞有急性杀伤作用。与单纯阿霉素组相比较,超声辐射增加了细胞内阿霉素的药物浓度,促进细胞凋亡的发生;但超声辐射没有影响细胞膜P-糖蛋白变化。结论:频率20kHz、声强0.25W/cm2、辐射时间60s的超声可以增强阿霉素对耐药细胞K562/A02的杀伤作用。BACKGROUND ＆ OBJECTIVE： Researches have shown that ultrasound can enhance the sensitivity of tumor cells towards chemotherapy drugs, thus to inhibit cell proliferation. This study was to investigate the antitumor effect of low-frequency ultrasound combined with adriamycin on human leukemia multidrug resistance （MDR） cell line K562/A02. METHODS. K562/A02 cells were divided into four groups： blank control group, adriamycin group, ultrasound group, and adriamycin plus ultrasound group. The trypan blue dye exclusion assay and MTT assay were used to determine the viability and proliferation of K562/A02 cells, while Wright＇s stain and flow cytometry were used to determine the apoptosis and the concentration of adriamycin. The expression of P-glycoproteins （P-gp） was detected using immunocytochemistry. RESULTS： Ultrasound （20 kHz, 0.25 W/cm2, 60s） combined with adriamycin （7.5 μg/mL） induced more apoptosis of K562/ A02 cells than adriamycin alone. Compared with the adriamycin group, ultrasound at a frequency of 20 kHz and an intensity of 0.5 W/cm2 exerted an acute killing effect on cells. Ultrasound increased the intracellular concentration of adriamycin and promoted apoptosis of K562/A02 cells, but did not change the expression of P-gp on the cell membrane. CONCLUSION： Ultrasound at a frequency of 20 kHz, an intensity of 0.25 W/ cm2 and duration of 60s can enhance the killing effect of adriamycin on K562/A02 cells.

关 键 词：超声 阿霉素 白血病 耐药细胞 凋亡 

分 类 号：R733.7[医药卫生—肿瘤]