两种fimA基因型牙龈卟啉单胞菌刺激下口腔上皮细胞ICAM-1的表达  

ICAM-1 Inductions of oral epithelial cells by Porphyromonas gingivalis with different fimA genotypes

作　　者：郭永华[1] 吴亚菲[2] 刘天佳[2] 肖晓蓉[3] 

机构地区：[1]首都医科大学附属北京口腔医院牙周粘膜科,北京100050 [2]四川大学华西口腔医学院口内教研室 [3]教育部口腔生物医学工程重点实验室

出　　处：《北京口腔医学》2008年第5期241-243,共3页Beijing Journal of Stomatology

基　　金：国家自然科学基金资助项目(No30471890)

摘　　要：目的比较2种fimA基因型牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)刺激下口腔上皮细胞ICAM-1的表达水平。方法实验组用P. gingivalis ATCC 33277(I型菌毛组),W83(IV型菌毛组),47A-1(IV型菌毛组)分别与KB细胞(ATCC CCL17)共同孵育24h;对照组为未受P. gingivalis刺激的KB细胞。分别在1h、3h、6h和24h收集细胞,运用流式细胞仪检测KB细胞膜上ICAM-1的动态表达。结果P. gingivalis刺激细胞后3h、6h和24h,实验组ICAM-1的表达水平均高于对照组;2种fimA基因型P. gingivalis调节KB细胞表达ICAM-1的方式相似,IV型菌毛组的调节作用强于I型菌毛组。结论P. gingivalis上调口腔上皮细胞表达ICAM-1的水平与其fimA基因型相关,提示P. gingivalis致病性与其fimA基因型相关。Objective To investigate the intercellular adhesion molecule 1 （ICAM-1） expression in oral epithelial cells by challenge of P. gingivalis with different fimA genotypes. Methods P. gingivalis ATCC 33277 （type Ⅰ）, W83 （type Ⅳ） and 47A-1 （type Ⅳ） were assessed for their inductions of ICAM-1 expression in the human oral epithelial ceils （KB cell line, ATCC CCL17）. KB cells without the stimulation of P. gingivalis were used as control. ICAM-1 expression in KB cell membrane was determined by flow cytometry （FCM） at different time intervals （ 1 h, 3 h, 6 h and 24 h） following continuous co-culture of bacteria with KB cell line. Results Expression of ICAM-1 was up-regulated when KB cells were co-cultured with P. gingivalis at 3 h, 6 h and 24 h. ICAM-1 expression way in oral epithelial cells by challenge of P. gingivalis with I fimA and IV fimA was similar, and strains with IV fimA were stronger than strains with I fimA. Conclusion fimA genotypes of P. gingivalis are related to the effect of ICAM-1 inductions, which indicates fimA genotype may be associated with pathogenesis of P. gingivalis.

关 键 词：牙龈卟啉单胞菌 FIMA基因型 细胞间黏附分子-1 

分 类 号：R780.2[医药卫生—口腔医学]