突变二氢叶酸还原酶基因转导及乳癌耐药细胞株的建立  

MUTANT DIHYDROFOLATE REDUCTASE GENE TRANSDUCTION AND ESTABLISHING THE DRUG RESISTANT CELL STRAIN OF BREAST CANCER

作　　者：王桂林[1,2,3,4] 吴恩[1,2,3,4] 许佐良 张莎莎[1,2,3,4] 爱民 侯明星[1,2,3,4] 

机构地区：[1]内蒙古医学院药理教研室 [2]内蒙古医学院中心实验室 [3]北京市肿瘤防治研究所 [4]内蒙古医学院第一附属医院外科

出　　处：《内蒙古医学院学报》1997年第4期8-11,共4页Acta Academiae Medicinae Neimongol

摘　　要：为了研究耐药肿瘤细胞的酶学动力学变化及寻找和筛选逆转耐药性的药物，用含有突变的二氢叶酸还原酶（ＤＨＦＲ）基因的高效反转录病毒上清感染人乳癌细胞ＭＤＡ２３１，经药物筛选，ＤＮＡ印迹法、ＲＮＡ印迹法及细胞对药物耐受性实验检测，确定ＤＨＦＲ基因已整合入细胞基因组ＤＮＡ中并表达，转染细胞对Ｇ４１８，ＭＴＸ有不同程度的耐受，成功地建立了一个耐受ＭＴＸ的乳癌细胞株，为进一步研究提供了实验材料。In order to study enzyme kinetics changes, characterization of resistant tumor cells, a model of drug resistant cells is established。Retrovirus supernatant containing a mutant dihydrofolate reductase(DHFR) gene infected human breast carcinoma cell, MDA-231 cells。Analysis using molecular hybridizations(Southern blotting, Northern blotting) showed that mutant DHFR gene integrated into genomic DNA and expressed。Celllular tolerant trial showed that the gene-modified tumor cells demonstrated different resistance to G418 and MTX。Hence, a MTX-resistance cell strain was established。The cell is useful for further studying the function of DHFR gene and use in clinical.

关 键 词：二氢叶酸还原酶 基因 乳癌细胞 MDA231 耐药性 

分 类 号：R737.902[医药卫生—肿瘤] R915[医药卫生—临床医学]