重组大肠杆菌厌氧发酵产丁二酸培养条件的优化  

Optimization of the Culture Conditions for Succinic Acid Production by Anaerobic Fermentation of Recombinant Escherichia coli

作　　者：姜岷[1] 王益娜[1] 陈可泉[1] 马江锋[1] 李建[1] 刘忠敏[1] 

机构地区：[1]南京工业大学制药与生命科学学院,材料化学工程国家重点实验室,江苏南京210009

出　　处：《食品与发酵工业》2008年第9期6-10,共5页Food and Fermentation Industries

基　　金：国家高科技发展计划"863"(No.2006AA02Z235);国家自然科学基金资助(No.20606017);国家自然科学基金重点项目(No.20336010);江苏省高校自然科学研究计划(No.05KJB180043)

摘　　要：采用Plackett-Burman设计法(Plackett-Burman,PB)对影响Escherichia coli NZN111(sfcA)厌氧发酵生产丁二酸的11个因子进行了筛选。结果表明,影响该菌厌氧发酵生产丁二酸的主要影响因子为乙酸钠、MgCO3、接种量、诱导剂IPTG加入时间和发酵周期。在此基础上采用响应曲面法(Response Surface Methodology,RSM)对这5个因子的影响进行了研究,得出多元二次回归方程拟合的5种因素与丁二酸产量间的函数关系,并根据优化结果与实验验证,当初始葡萄糖浓度为22 g/L,乙酸钠1 g/L,MgCO317 g/L,接种量10%,IPTG初始时加入,发酵周期76 h时,丁二酸产量从原始发酵培养条件下的11.84 g/L提高到15.64 g/L,收率从53.8%提高到71.1%。Plackett-Burman design was used to evaluate the effects of eleven factors for succinic acid production by Escherichia coli NZN111（sfcA）. The results showed the following key factors： sodium acetate, magnesium carbonate, inoculum size, time length of adding IPTG and incubation period. Based on these resuits, response surface methodology （RSM） was applied for further optimizing these parameters in order to find out the optimum concentration levels. The concentration of succinic acid could reach 15.64g/L, compared to the initial 11.84 g/L, and the yield was enhanced from 53.8% to 71.1% with the initial glucose concentration of 22 g/L, 1 g/L of acetate, 17 g/L of magnesium carbonate, 10 % of inoculum size, IPTG initially added and incubation period of 76 h.

关 键 词：E.COLI NZN111 丁二酸 Plackett-Burman设计法 响应曲面法 

分 类 号：TQ921.4[轻工技术与工程—发酵工程]