双水相系统微生物转化植物甾醇制备雄烯二酮研究  

Study on Biotransformation of AD from Phytosterol in Two-Phase Systems

作　　者：杨英[1,2] 姜绍通[1] 潘丽军[1] 易守连[1] 

机构地区：[1]合肥工业大学生物与食品学院,安徽合肥230009 [2]安徽建筑工业学院环境工程系,安徽合肥230022

出　　处：《食品与发酵工业》2008年第9期61-64,共4页Food and Fermentation Industries

基　　金：国家“十一五”科技支撑计划项目(2006BAD05A12);安徽省自然科学基金项目(070413265)

摘　　要：研究了高聚物/无机盐双水相系统、高聚物/高聚物双水相系统对转化的影响;高聚物/高聚物总浓度对甾醇转化率的影响。试验结果表明,不同高聚物组成的双水相系统都可以满足将甾醇转化为AD的要求,而且明显比在高聚物与无机盐双水相系统中转化率高;总浓度20%时转化率较高,且PEG10000和DEX20000的浓度分别为12%和8%时,转化率达到了50%左右,较浓度均为10%时提高了5%。A series of study on biotransformation of AD by Mycobacterium sp-uv-8 was conducted by transforming the fermentation system. The effects of polymer-salt two-phase systems and different polymer-polymer two-phase systems on bioconversion were studied. At the same time, the effect of the general con- centration of polymer-polymer on bioconversion and the rate of bioconversion in polymer-polymer two-phase systems is higher than in polymer-salt two-phase systems. When the general concentration is 20%, the rate of bioconversion is higher; when the concentration of PEG10000 and DEX20000 are12% and 8%, respectively, the rate of bioconversion is up to 50%. AD transformation productivity was 5% higher than that of 10% concentration respectively.

关 键 词：雄烯二酮 双相系统 植物甾醇 生物转化 

分 类 号：TS201.3[轻工技术与工程—食品科学]