小扁豆种质资源SSR标记遗传多样性及群体结构分析  

Analysis of Genetic Diversity and Population Structure in Lentil(Lens cu-linaris Medik.) Germplasm by SSR Markers

作　　者：刘金[1] 关建平[1] 徐东旭 张晓艳[1] 顾竟[1] 宗绪晓[1] 

机构地区：[1]中国农业科学院作物科学研究所／国家农作物基因资源与基因改良重大科学工程,北京100081 [2]张家口市农业科学院作物所,河北张家口075131

出　　处：《作物学报》2008年第11期1901-1909,共9页Acta Agronomica Sinica

基　　金：国家“十一五”科技支撑计划项目(2006BAD13B05,2006BAD02B08);科技部植物种质资源共享平台建设项目(2005DKA21001-06);农业部作物种质资源保护项目[NB07-2130135-(25-30)-13];农业行业标准专项(nyhyzx07-017);中澳政府间大型双边国际合作项目(ACIAR:CS1/2000/035)

摘　　要：从145对SSR引物中筛选到14对多态性引物,对选取国家种质库的440份小扁豆种质资源进行SSR标记遗传多样性分析,共检测出87个等位变异,平均每个SSR位点6.2143个;平均Shannon-Weaver指数(I)为1.1869。16个不同地理来源群体间表现出显著的遗传多样性差异,国外群体的遗传多样性水平(0.9837)远高于国内群体(0.3485)。PCA、UPGMA法聚类分析和Structure群体结构分析结果相互间完全吻合。440份参试材料从遗传结构上可划分为8个组群,揭示国外群体遗传分化大,群体间的亲缘关系较远;国内群体与之相反。研究结果显示,山西、宁夏和甘肃省是我国小扁豆资源遗传多样性最丰富、遗传关系较复杂的地区,应对该区域小扁豆资源进一步搜集、保护和研究;同时,应继续加强小扁豆资源的国外引种与交流,做进一步系统研究和开发利用。A total of 440 lentil accessions, randomly chosen from the National Gene Bank, were employed in the experiment. Fourteen polymorphic SSR markers was screened from a total of 145 SSR primer pairs, detected a total of 87 allelic variations. High level of diversity has been detected, with an average allele number of 6.2143 and an average Shannon-Weaver index （/） of 1.1869 by 14 S SR primer pairs. The results indicated significant differentiations among the 16 groups of lentil resources in genetic diversity from diversed geographic origins. The genetic diversity within alien accessions was obviously higher than that within Chinese resources. Population structure within the subcollection of 440 individuals was defined, and 8 clusters were identified based on SSR markers by using Structure 2.2 software. The results of PCA, UPGMA cluster and population structure analyses revealed that the genetic structure was multiplex; the genetic distance was broader among foreign populations than that among Chinese populations. More survey and collecting trips, conservation and study should be made for the lentil genetic resources in Shanxi, Ningxia, and Gansu provinces, where the highest genetic diversity level existed in China. Furthermore, international exchange and introduction of lentil genetic resources should be enhanced, for more systematic studies and exploitation.

关 键 词：小扁豆 种质资源 SSR标记 遗传多样性 群体结构 

分 类 号：S529[农业科学—作物学]