外用表没食子儿茶素没食子酸酯抵抗中波紫外线对小鼠皮肤的慢性光损伤作用  

Protective effect of topical (-)-epigallocatechin-3-gallate against chronic photodamage induced by UVB irradiation on the skin of BALB/C mouse

作　　者：徐丽贤[1,2] 骆丹[1] 徐晶[1] 林向飞[1] 吉玺[1] 朱洁[1] 吴迪[1] 李巍[3] 

机构地区：[1]南京医科大学第一附属医院皮肤性病科,江苏南京210029 [2]江苏省省级机关医院皮肤科,210024 [3]扬州大学医学院病理教研室,江苏扬州225001

出　　处：《临床皮肤科杂志》2008年第11期695-698,共4页Journal of Clinical Dermatology

基　　金：国家自然科学基金资助项目(30371294)

摘　　要：目的:研究中波紫外线(UVB)慢性辐射BALB/C小鼠后皮肤的组织病理改变、表皮细胞凋亡情况及表没食子儿茶素没食子酸酯(EGCG)对其的影响。方法:EGCG局部外用于小鼠耳、背部皮肤后分别给予不同强度的UVB辐射,每日1次,连续1个月,石蜡切片苏木精-伊红染色观察不同处理条件下皮肤组织病理变化,同时应用末端转移酶介导的缺口末端标记(TUNEL)法检测小鼠表皮中的凋亡细胞。结果:UVB慢性辐射对BALB/C小鼠皮肤有明显影响,主要表现有表皮过度角化、棘层肥厚、海绵样水肿、晒斑细胞、真皮乳头层水肿、毛细血管扩张、炎性细胞浸润等。EGCG预处理能减轻UVB诱导的上述组织病理变化。另外,对慢性低剂量UVB辐射组,EGCG有一定的促细胞凋亡作用。结论:不同剂量UVB慢性辐射后小鼠皮肤组织病理改变明显,EGCG可保护UVB辐射诱导的光损伤作用,并对低剂量慢性UVB辐射后BALB/C小鼠皮肤细胞有促进凋亡作用。Objective： To investigate the pathological changes and the apoptosis of epidermal cells on the skin of BALB/C mouse induced by chronic ultraviolet B （UVB） irradiation and the photoprotective effect of topical application with （-）-epigallocatechin-3-gallate （EGCG） against it. Methods： The skin of the ears and the back of BLAB/C mice was pretreated with EGCG and then irradiated with different doses of UVB daily for one month. The paraffin sections of skin specimens were stained with hematoxylin-eosin stain for observing the pathological changes. The apoptotie cells were detected by TUNEL. Results： There were obvious pathological changes in the skin of BALB/C mouse after chronic UVB irradiation, they were hyperkeratosis, acanthosis, spongiosis, sunburn cells in the epidermis, edema in the papillary dermis, telangiectasia and inflammatory cell infiltration in the dennis. These UVB-induced histopathological changes could be reduced by the pre-intervention with EGCG. In the group of low-dose-UVB irradiation, enhanced apoptosis by EGCG was observed. Conclusions： There are marked histopathological changes in the skin of BALB/C mice after the chronic UVB irradiation with different doses of UVB. Topical application of EGCG on the skin of BALB/C mice can prevent UVB-induced photodamage, and promote the epidermal cell apoptosis induced by the low dose irradiation of UVB.

关 键 词：表没食子儿茶素没食子酸酯 紫外线 中波 BALB/C小鼠 

分 类 号：Q682[生物学—生物物理学]