蛇毒神经生长因子对大鼠视网膜缺血再灌注损伤后微管相关蛋白1B的影响  

The effects of viper venom nerve growth factor on the expression of MAPIB on experimental retinal ischemia/reperfusion injury

作　　者：王瑛[1] 刘金华[1] 唐秀武[2] 

机构地区：[1]广西医科大学第一附属医院眼科中心,南宁530021 [2]广西壮族自治区民族医院眼科

出　　处：《中国实用眼科杂志》2008年第11期1278-1280,共3页Chinese Journal of Practical Ophthalmology

基　　金：本课题受国家自然科学基金资助（资助号：30460138）

摘　　要：目的探讨玻璃体腔内注射蛇毒神经生长因子是否对实验性大鼠视网膜缺血再灌注损伤的视网膜中的微管相关蛋白1B有影响而起到神经保护作用。方法采用升高大鼠跟内压的方法，制作实验性视网膜缺血再灌注损伤模型。实验组和对照组分别注入蛇毒神经生长因子和平衡盐溶液，通过免疫组织化学法检测再灌注后不同时间段各组大鼠视网膜组织中微管相关蛋白1B的表达。结果微管相关蛋白1B的表达在实验组于再灌注后6h加强，48h达高峰。而对照组在再灌注后24h增强，96h才达高峰，而且高峰浓度实验组较对照组强（P〈0．01）。结论通过向大鼠玻璃体腔内注射蛇毒神经生长因子可以提高视网膜微管相关蛋白1B的表达而对实验性视网膜缺血再灌注损伤有神经保护作用。Objective To evaluate the effects of viper venom nerve growth factor （vNGF） on the expression of Microtubule associated proteins 1B （MAP1B） after vNGF was injected into the vitreous cavity on experimental retinal ischemia/reperfusion injury （RIR）.Method The Spregue-Dawley （SD） rat model of RIR was made by increasing the intraocular pressure.The rats were divided into normal,experimental and control groups randomly.At the beginning of reperfusion,20μl （100BU） of vNGF was injected into the experimental group and 20μl of Balanced Salt Solution （BSS） was injected into the vitreous cavity in Control group.The expression of MAP1B in retina at different time after reperfusion were observed.The expression of MAPIB was studied by immunohistochemistry.Result The expression of MAP1B was found in normal group.In experimental group the expression of MAP 1B enhanced gradually at 6h after reperfusion, reached the peak at 48h.In Control group, the expression of MAP1B enhanced gradually at 24h after reperfusion, reached the peak at 96h, and at the same time the light density of stain was more weak than that at 48h in experimental group （ P 〈0.01 ）.The expression of MAP 1B of experimental and control group decreased at 120h, and then the expression of MAP1B at 168h was similar as normal group.Conclusion Injection ofvNGF into the vitreous cavity has the protection effect on experimental retinal ischemia/reperfusion injury through enhancing the expression of MAP 1B to promote the regeneration of retinal nerve fiber.

关 键 词：缺血再灌注损伤 蛇毒神经生长因子 微管相关蛋白1B 

分 类 号：R774.1[医药卫生—眼科] R651.3[医药卫生—临床医学]