Ang-1,Ang-2和Tie2基因在骨肿瘤组织中的差异表达  

Differential Expression of Ang-1,Ang-2 and Tie2 mRNA in Bone Tumors

作　　者：李皓桓[1] 彭昊[1] 方洪松[1] 汤洁[1] 温志远[1] 

机构地区：[1]武汉大学人民医院骨科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2008年第6期780-782,791,共4页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:30600627);武汉市青年科技晨光计划资助项目(编号:200750731256)

摘　　要：目的:探讨Ang-1、Ang-2及Tie2 mRNA在骨肿瘤组织中的表达及其意义。方法:采用逆转录聚合酶链反应法对正常骨组织、骨软骨瘤、尤文肉瘤和骨肉瘤组织标本各6例内Ang-1、Ang-2及Tie2 mRNA含量进行半定量检测。结果:恶性骨肿瘤组织(尤文肉瘤、骨肉瘤)内Ang-2 mRNA/Ang-1 mRNA比值较正常骨组织和良性肿瘤骨组织比值上调。尤文肉瘤和骨肉瘤中Tie2 mRNA的表达上调。结论:恶性骨肿瘤组织内Ang-1,Ang-2及Tie2mRNA表达失衡,肿瘤骨组织内可能存有Ang/Tie2的自分泌和旁分泌系统。深入探讨Ang-1/Ang-2/Tie 2在肿瘤骨组织中的分子生物学机制及其临床病理联系,有望使其成为判定骨肿瘤预后的指标,并为骨肿瘤分子治疗提供靶位点。Objective： To investigate the expression of Ang-1, Ang-2 and Tie2 mRNA in bone tumor tissues and to explore its clinical significance. Methods： Semi-quantative RT-PCR was used to analyze the expression of Ang-1, Ang-2 and Tie2 mRNA in tissues harvested from normal bone, osteochondroma, Ewing＇s sarcoma and osteosarcoma（n=6）. Results： Ratio of Ang-2 mRNA/Ang-1 mRNA was increased in malignant lesions （Ewing＇s sarcoma and osteosarcoma） when compared with benign lesions （normal bone and osteochondroma）. Expression of Tie2 mRNA was up-regulated in Ewing＇ sarcoma and osteo- sarcoma. Conclusion. The balance of Ang-1, Ang-2 and Tie2 mRNA levels was disturbed in malignant lesions. There may exist a possible autocrine and paracrine pathway of Angs/Tie2 system in bone malignant lesions. Further investigation of Ang1-, Ang-2 and Tie2 and their biomedical significance in bone tumor tissues and clarify their clinical-pathological relation will make them possible prognostic indices and provide molecular therapy target.

关 键 词：血管生成素 骨肿瘤 逆转录聚合酶链反应 

分 类 号：R738.1[医药卫生—肿瘤]