白藜芦醇对人宫颈癌C33A,SiHa和HeLa细胞增殖与凋亡的影响  

Effects of Res on proliferation and apoptosis of human cervical carcinoma cell lines C33A,SiHa and HeLa

作　　者：申新[1] 于良[2] 张靖[3] 李胜男[3] 潘承恩[2] 

机构地区：[1]西安交通大学医学院第一附属医院麻醉科,陕西西安710061 [2]西安交通大学医学院第一附属医院肝胆外科,陕西西安710061 [3]西安交通大学医学院第一附属医院妇产科,陕西西安710061

出　　处：《第四军医大学学报》2008年第18期1658-1661,共4页Journal of the Fourth Military Medical University

基　　金：陕西省自然科学基金(2002C249)

摘　　要：目的:探讨白藜芦醇(Res)对宫颈癌C33A,SiHa和HeLa细胞增殖与凋亡的影响.方法:MTT法检测不同浓度Res对C33A,SiHa和HeLa细胞的抑制率.流式细胞仪检测细胞的凋亡率及细胞周期.荧光显微镜观测凋亡细胞.结果:Res在一定浓度范围内,以浓度和时间依赖的方式抑制宫颈癌C33A,SiHa和HeLa细胞的生长(P<0.01).荧光显微镜下细胞呈现典型的凋亡性改变.Res对细胞周期无明显影响.结论:Res通过诱导细胞凋亡以时间依赖和浓度依赖的方式抑制宫颈癌C33A,SiHa和HeLa细胞的生长.AIM： To explore the effects of Res on the proliferation and apoptosis of human cervical cancer cell lines C33A, SiHa and HeLa. METHODS ： Inhibition rates of human cervical cancer cell lines C33A, SiHa and HeLa by different concertrations of Res were checked by MTT method. Apoptosis rates and cell cycles were checked by flow cytometry. Morphologic configuration of apoptotic cells were observed by fluorescence microscope. RESULTS： The growth of cervical cancer cell lines C33A, SiHa and HeLa were inhibited by Res in variable concentrations in a time- and dosage- dependent manner（P 〈 0. 01 ）. The cells showed characteristic apoptosis morphologic changes under fluorescence microscope. Res exerted no effects on cell cycles. CONCLUSION： Res inhibits the growth of cervical cell lines C33A, SiHa and HeLa by inducing cell apoptosis in time and dosage dependent

关 键 词：白藜芦醇 宫颈肿瘤 细胞凋亡 细胞增殖 

分 类 号：R737.33[医药卫生—肿瘤]