壶腹周围肿瘤染色体9p21缺失图谱的构建  

Deletion mapping on chromosome 9p21 in periampullary neoplasms

作　　者：王纯巍[1] 陆星华[2] 刘国仰[3] 戴理[3] 钱家鸣[2] 

机构地区：[1]空军总医院感染内科,北京100036 [2]中国医学科学院北京协和医院消化内科,北京100730 [3]中国医学科学院基础医学研究所医学遗传学研究室,北京100730

出　　处：《第四军医大学学报》2008年第18期1689-1691,共3页Journal of the Fourth Military Medical University

摘　　要：目的:进一步限定壶腹周围肿瘤染色体9p21区域的缺失范围.方法:选择染色体9p21区域5个微卫星多态性标记,通过聚合酶链反应、聚丙烯酰胺凝胶电泳和银染法,检测35例壶腹周围肿瘤及其外周血杂合性丢失(LOH)状况.结果:50%(4/8)胰腺癌有至少一个微卫星位点的LOH,其中D9S974(37.5%)和D9S942(28.6%)丢失频率较高,并且有连续性丢失现象.62.5%(5/8)壶腹癌在部分或全部位点出现LOH,其中D9S942(42.9%)丢失频率最高,其次为IFNA(37.5%)和D9S171(37.5%).14.2%(1/7)胰岛素瘤有一个位点LOH.结论:壶腹周围肿瘤染色体9p21最小共同缺失区位于D9S974和D9S942位点之间,其中可能存在一个新的涉及该肿瘤发生的相关抑癌基因.AIM： To further define the extent of chromosome 9p21 deletion region in human periampullary neoplasms. METHODS： The loss of heterozygosity （LOH） at 5 microsatellite polymorphic markers on chromosome 9p21 was detected by polymerase chain reaction, polyacrylamide gel electrophoresis and silver staining in 35 specimens of periampullary neoplasms and their matching blood samples. RESULTS ： Fifty percent （4/8） of pancreatic cancers showed the LOH at one or more microsatellite loci, with the more frequent sites of D9S974 （37.5%） and D9S942 （28.6%）, and some showed consecutive allelic loss, and 62.5% （5/8） of ampullary carcinomas showed LOH at partial or total of the loci, with the most frequent site of D9S942 （42.9%）, followed by IFNA （37.5%） and D9S171 （37.5%）. Loss of one locus was observed in 14.2% （1/7） of insulinoma. CONCLUSION： The minimal common region of chromosome deletion in periampullary neoplasms is defined between the D9S974 and D9S942 loci interval in 9p21, suggesting the involvement of a novel tumor suppressor gene in their carcinogenesis.

关 键 词：肝胰管壶腹 胰腺肿瘤 染色体 人 9对 染色体图 遗传标记 

分 类 号：R573[医药卫生—消化系统]