MiR-181c对人脑胶质瘤细胞增殖及凋亡的作用  

Effects of miR-181c on cell proliferation and apoptosis of human brain glioma cells

作　　者：刘书芳[1] 胡锦[2] 董万利[1] 柏燕燕[1] 金由辛[3] 

机构地区：[1]苏州大学附属第一医院神经内科,215006 [2]上海交通大学附属第六人民医院神经外科 [3]中国科学院生化所分子生物学国家重点实验室

出　　处：《神经疾病与精神卫生》2008年第5期335-337,共3页Journal of Neuroscience and Mental Health

基　　金：国家自然科学基金(编号:30371459);上海市科学技术发展基金(编号:034047)资助

摘　　要：目的观察miR-181c对人脑胶质瘤细胞增殖及凋亡的影响,进一步探讨miR-181c的生物学功能。方法化学合成miR-181c,脂质体转染U251细胞。应用cck-8法和流式细胞术检测细胞的增殖和凋亡。结果miR-181c上调后对U251细胞具有抑制作用,且呈明显的剂量依赖性,而对照组无明显抑制作用。结论化学合成的miR-181c在人脑胶质瘤细胞增殖和凋亡过程中发挥重要作用,可能成为胶质瘤基因治疗的新靶点。Objective To explore the biological function of miR--181c, and observe the effects of miR--181c on cell proliferation and apoptosis of human brain glioma cells. Methods MiR--181c was synthesized and transfected into the U251 malignant glioma cells by liposome. Cell viability was assessed by cck--8 assay. Cell apoptosis was detected by flow cytometry. Results MiR--181c inhibited U251 cell proliferation in a dose dependent manner, while the control group had no obvious effect on cell growth. Conclusions The chemically synthesized miR--181c plays an important role in cell proliferation and apoptosis of human brain glioma cells, which may be a promising target for glioma gene therapy.

关 键 词：微RNAMiR-181c 胶质瘤 细胞凋亡 

分 类 号：R739.4[医药卫生—肿瘤]