冠毒素高产菌株的半推理选育  

Half-rational Screening of Mutant Overproducing Coronatine

作　　者：吴晓玉[1,2] 储炬[1] 王园秀[2] 

机构地区：[1]华东理工大学生物反应器国家重点实验室,上海200237 [2]江西农业大学生物科学与工程学院,江西南昌330045

出　　处：《江西农业大学学报》2008年第5期888-893,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(30360050);江西省自然科学基金项目(0230043)资助

摘　　要：根据菌株利用萘为碳源与冠毒素生物合成的调控均受低温影响的特点,以及菌株对红霉素耐药机制受细胞膜孔蛋白和外排泵影响的特性,分别以菌株对萘代谢的温度敏感性及对红霉素抗性为筛选标志,依次采用亚硝基胍、5-溴尿嘧啶、紫外线、吖啶橙与紫外线混合等诱变方式,经系列推理选育,获得产冠毒素能力为100μg/mL的突变菌株Y57,比野生菌株BCC933产量提高7.3倍。On basis of screening labels of temperature - sensitivity of naphthalene metabolism and erythromycin resistance, the high -yield mutant Y57 was screened and obtained after the wild type strain BCC933 was treated with chemical and physical reagents such as NTG, 5 - BU and UV as well as acridine orange integrated with UV. According to the reported results ,both of naphthalene metabolisms as carbon source and coronatine biosynthesis in bacteria were sensitive to low temperature, and the resistance to erythromycin in bacteria resulted from effects of porins in cell membrane and efflux pump. Mutant Y57 producing coronatine 100 μg/mL was achieved in shake flask culture, which was nearly 7.3 times higher than that of wild strain.

关 键 词：突变菌株 冠毒素 诱变剂 红霉素 萘 

分 类 号：Q93-33[生物学—微生物学]