健骨汤对去睾丸大鼠松质骨的影响  

The Influence of Jiangu Decoction on Cancellous Bone in Rats after Orchiectomy

作　　者：代洪宾[1,2] 李飞跃[2] 罗仕华[2] 张凤华[2] 张伟滨[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院骨科,上海200025 [2]上海市伤骨科研究所

出　　处：《中国中医骨伤科杂志》2008年第11期16-18,共3页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：上海交大医学院科技基金项目(06XJ21028)~~

摘　　要：目的:运用骨密度、骨生物力学检测和Micro CT分析及三维结构重建的方法,观察健骨汤对老龄去势大鼠松质骨的影响。方法:30只12月龄雄性wistar大鼠随机分为ORX组、Sham组、ORX加健骨汤(ORX+JG)组,每组10只。前两组生理盐水灌胃每日,ORX+JG组用健骨汤提取液(5ml/kg/d))灌胃,12周后取材.运用双能X线骨矿含量测定仪(DXA)检测治疗后大鼠全身总骨密度;取L3椎体、右股骨行MicroCT分析及三维结构重建,取L4椎体、左股骨行骨生物力学测试。结果:与去势组比较,ORX+JG组BMD、骨小梁体积(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数目(Tb.N)值升高,骨生物力学检测发现骨最大载荷(M-load)、吸收能量(Enery)、最大应力(M-stress)、弹性模量(E-lastic)值升高,骨小梁间隙(Tb.Sp)、结构模型指数(SMI)值降低。结论:健骨汤能明显改善去势大鼠骨微结构,增加骨强度和骨密度,防止骨量丢失。Objective：To investigate the influence of Jiangu Decoction （JGD） on cancellous bone in senile orchiectomized （ORX） rats via bone mineral density （BMD） test, bone biomechanical analysis, micro--CT scanning and three--dimensional structure reconstruction. Methods： Thirty 12--month--old male Wister rats were randomly divided into ORX group, sham--operated group （Sham group） and ORX＋JGD group, 10 rats in each group. Rats in Sham group and ORX group received intragastric administration of 0, 9% NaCl daily while rats in ORX＋JD group received JGD extract （Sml/kg/d） for 12 weeks. Dual energy X--ray absorptiometry was used to measure total body BMD, micro--CT scanning and three--dimensional structure reconstruction were used to analyze right femurs and L-3 vertebra, and bone biomechanical test was carried on left femurs and L-4 vertebra. Results： Compared with ORX group, the following indexes in ORX＋JGD all significantly increased： BMD, the volume, thickness and number of trabeeular bone, maximum load, energy absorption, maximum stress and elastic modulous. Meanwhile, the space of trabecular bone and structure model index all decreased. Conclusion：Jiangu Decoction could prevent the bone loss, increase micro-architecture and biomeehanical properties of the trabecular bone in ORX rats.

关 键 词：健骨汤 骨密度 MICRO CT 骨生物力学 

分 类 号：R-33[医药卫生]