γ-干扰素对胃癌细胞TNF受体表达和TNF突变体代谢的影响  

The effect of γ interferon on receptor and cytotoxic response of tumor necrosis factor mutant

作　　者：冉瑞琼[1] 吴裕炘[2] 孙建中[4] 朱承谟[2] 王国平[3] 唐国忠[3] 曹世龙[1] 

机构地区：[1]上海医科大学附属肿瘤医院流式细胞分析室 [2]上海第二医科大学附属瑞金医院 [3]中国科学院上海原子核研究所 [4]美国南加州大学医学院免疫学系

出　　处：《中华核医学杂志》1997年第4期201-203,共3页Chinese Journal of Nuclear Medicine

摘　　要：为探讨γ干扰素（ＩＦＮ）对胃癌细胞（ＳＧＣ７９０１）肿瘤坏死因子（ＴＮＦ）受体的影响及与肿瘤坏死因子突变体（ＴＮＦｍ）协同抗肿瘤的机制，以１２５ＩＴＮＦｍ为配体，用放射受体分析法研究γＩＦＮ对ＳＧＣ７９０１细胞ＴＮＦ受体表达及与ＴＮＦｍ内化和降解的影响。并用ＭＴＴ法研究γＩＦＮ与ＴＮＦｍ协同抗肿瘤作用。结果：γＩＦＮ使ＳＧＣ７９０１细胞ＴＮＦ受体的数量由０５６×１０－１１ｎｍｏｌ／细胞增至０９４×１０－１１ｎｍｏｌ／细胞，而对受体的亲和力无影响（Ｋｄ为２７８×１０－１１和２６４×１０－１０ｍｏｌ／Ｌ，ｔ＝１２，Ｐ＞０５）；γＩＦＮ可明显增加ＴＮＦｍ内化和降解的绝对数量，而对其比率无影响。γＩＦＮ使ＴＮＦｍ对ＳＧＣ７９０１细胞的最大杀伤率达到９６％（ｔ＝５２，Ｐ＜００１）。提示：γＩＦＮ通过上调ＴＮＦ受体而加强ＴＮＦｍ的体外抗肿瘤作用。PURPOSE To explore the effect of γ interferon on tumor necrosis factor receptor (TNFR) of cultured human gastric cancer cells (SGC 7901 ) and the mechanism of anti tumor synergism of γ interferon and tumor necrosis factor mutant (TNF m). METHODS The 125 I tumor necrosis factor mutant ( 125 I TNF m) was used as radioligand. The radioassay was applied to detect the effect of γ interferon on the expression of TNFR of SGC 7901 cells and on the internalization and degradation of TNF m. MTT colorimetric methed was used in studying the anti tumor synergism of γ interferon and TNF m. RESULTS By virtue of γ interferon, the amount of TNFR of SGC 7901 cells was increased from 0 56×10 -11 to 0 94×10 -11 nmol/cell. No effect was shown on the affinity of the receptors (Kd value: 2 78×10 -10 mol/L versus 2 64×10 -10 mol/L, t=1 2, P>0 5). γ interferon could promote the synthesis of TNFR protein; and also markedly increase the absolute amount of the internalization and degradation of TNF m, but exerted no effect on their ratios. The maximum cancer cell cytosidal rate of TNF m on SGC 7901 was 96% (t=5 2,P<0 01) under the influence of γ interferon. CONCLUSIONS [WT5”BZ]γ interferon increased the anti tumor effect in vitro of TNF m by up regulating the TNFR.

关 键 词：Γ-干扰素 胃肿瘤 胃癌细胞 肿瘤坏死因子 受体 

分 类 号：R735.2[医药卫生—肿瘤]