双生病毒的同义密码子用法及其进化分析  

Analysis on synonymous codon usage and evolution in geminiviruses

作　　者：徐晓忠[1] 刘庆坡[2] 樊龙江[1] 周雪平[1] 

机构地区：[1]浙江大学农业与生物技术学院,杭州310029 [2]浙江林学院农业与食品科学学院,临安311300

出　　处：《植物病理学报》2008年第5期478-488,共11页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30530520);高等学校科技创新工程重大项目培育资金资助项目(705025)

摘　　要：对双生病毒编码基因的变异进行了密码子用法分析,推测双生病毒基因的表达水平普遍不高,而外壳蛋白基因的表达水平相对较高,它们在密码子第3位上偏好使用A或T。双生病毒的密码子用法主要受到突变偏好和翻译选择等因素的影响。双生病毒的密码子使用具有基因特异性和一定程度的寄主特异性。基于相对同义密码子使用频率的聚类分析可以很好反映侵染单子叶植物和双子叶植物双生病毒之间的差异,也在一定程度上反映出双生病毒之间的亲缘关系。Synonymous codon usage variation had been investigated in protein-coding genes of geminiviruses.Most of the genes were speculated to be lowly expressed,but coat protein（CP）gene was considered as relatively highly expressed one,which preferred to use those codons ending with A or T.The codon usage variation in geminiviruses was influenced by several factors such as mutation bias and translational selection.The codon usage patterns in geminiviruses were gene-specific and certain host-specific.Cluster analysis based on relatively synonymous codon usage（RSCU）could efficiently and effectively reflect the difference between the monocot-infecting and dicot-infecting geminiviruses,and to a certain extent reflect the evolutionary relationship among them.

关 键 词：双生病毒 密码子用法 相对同义密码子使用频率 聚类分析 进化 

分 类 号：S432.41[农业科学—植物病理学]