南瓜粉的功能研究  

Study on Functional Properties of Pumpkin Powder

作　　者：李筱泉[1] 何群[1] 练惠辉[1] 王勤[1] 

机构地区：[1]厦门大学生命科学学院,教育部细胞生物学与肿瘤细胞工程重点实验室,福建厦门361005

出　　处：《食品科学》2008年第10期55-58,共4页Food Science

基　　金：福建省自然科学基金项目(B0510001)

摘　　要：本实验用中性蛋白酶酶解制得的南瓜粉,通过测定该南瓜粉对ACE和ALP活力的影响,研究了南瓜粉对血管紧张素转化酶(angiotension converting enzyme,ACE)和碱性磷酸酶(alkaline phosphatase,ALP)活性的影响,考察其降血压作用及潜在的抗肿瘤作用;同时提取并初步纯化南瓜多糖,通过fenton反应产生羟基自由基(·OH),测定南瓜粉清除·OH能力,以及通过FRAP法测定其总还原能力,利用纸层析法分析南瓜多糖的单Z糖组成。结果表明,该南瓜粉对ACE和ALP均有抑制作用,具有一定的降血压作用和潜在的抗肿瘤作用;南瓜多糖是由半乳糖、葡萄糖、阿拉伯糖和鼠李糖四种单糖组成,具有良好的抗氧化活性。Pumpkin powder was produced by using neutral proteinase to hydrolyze pumpkin pulp and then freeze-drying, and pumpkin polysaccharides were extracted. In order to understand the hypotensive effect and potential antitumor effect of the pumpkin powder, its effects on the activities of ACE （angiotensin converting enzyme） and ALP （alkaline phosphatase） were investigated. The scavenging effect of the pumpkin powder was determined on hydroxl radical （oH）, which is produced by Feton reaction, and its total reducing power was determined by FRAP method. The monosaccharide composition of the pumpkin polysaccharides was analyzed by paper chromatography method. The results showed that the pumpkin powder can inhibit the activities of ACE and ALP and has hypotensive effect and potential antitumor effect. The pumpkin polysaccharides have antioxidant activity, which are composed of galactose, glucose, arabinose and rhamnose.

关 键 词：南瓜 ACE ALP 多糖 抗氧化 

分 类 号：Q356.1[生物学—遗传学]